SIRT6 Human
Sirtuin-6 Human Recombinant
Cat. No.
BT28152
Source
Escherichia Coli.
Synonyms
Mono-ADP-ribosyltransferase sirtuin-6, SIR2-like protein 6, SIRT6, SIR2L6, Sirtuin-6.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SIRT6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 375 amino acids (1-355 a.a.) and having a molecular mass of 41 kDa. Recombinant SIRT6 is fused to 20 amino acids His-tag at N-terminus and purified by conventional chromatography techniques.
Product Specs
Introduction
Belonging to the sirtuin family of proteins (Class IV), SIRT6 is homologous to the yeast Sir2 protein and possesses a sirtuin core domain. Yeast sirtuin proteins are known for their ability to regulate epigenetic gene silencing and suppress rDNA recombination. As a chromatin-associated protein, SIRT6 plays a role in DNA repair. Human sirtuins act as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
Description
Recombinant SIRT6 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 375 amino acids (1-355 a.a.). With a molecular mass of 41 kDa, it features a 20-amino acid His-tag fused at the N-terminus. Purification is achieved through standard chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The SIRT6 protein solution (1 mg/ml) is formulated in 20 mM Tris-HCl buffer at pH 8 with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Mono-ADP-ribosyltransferase sirtuin-6, SIR2-like protein 6, SIRT6, SIR2L6, Sirtuin-6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSVNYAAGLS PYADKGKCGL PEIFDPPEEL ERKVWELARL VWQSSSVVFH TGAGISTASG IPDFRGPHGV WTMEERGLAP KFDTTFESAR PTQTHMALVQ LERVGLLRFL VSQNVDGLHV RSGFPRDKLA ELHGNMFVEE CAKCKTQYVR DTVVGTMGLK ATGRLCTVAK ARGLRACRGE LRDTILDWED SLPDRDLALA DEASRNADLS ITLGTSLQIR PSGNLPLATK RRGGRLVIVN LQPTKHDRHA DLRIHGYVDE VMTRLMEHLG LEIPAWDGPR
VLERALPPLP RPPTPKLEPK EESPTRINGS IPAGPKQEPC AQHNGSEPAS PKRERPTSPA PHRPPKRVKA KAVPS.
VLERALPPLP RPPTPKLEPK EESPTRINGS IPAGPKQEPC AQHNGSEPAS PKRERPTSPA PHRPPKRVKA KAVPS.
Product Science Overview
Structure and Function
Biological Processes
SIRT6 is involved in several biological processes, including:
- DNA Repair: It plays a significant role in the repair of double-strand breaks via homologous recombination .
- Telomere Maintenance: SIRT6 is essential for maintaining telomere integrity, which is crucial for cellular aging and longevity .
- Glycolysis Regulation: It negatively regulates the glycolytic process, thereby influencing glucose homeostasis .
- Inflammation: SIRT6 has anti-inflammatory properties, which help in reducing age-related disorders and premature aging .
Therapeutic Potential
Human sirtuins, including SIRT6, are considered potential therapeutic targets for a broad spectrum of diseases such as cancer, neurodegenerative diseases, and metabolic disorders . Despite the challenges in developing isoform-selective and potent molecules with good drug-like properties, small molecule sirtuin regulators have advanced into clinical trials .
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