SIRT1 Human
Sirtuin-1 Human Recombinant
Cat. No.
BT27813
Source
Escherichia Coli.
Synonyms
Sirtuin 1, SIRT1, SIR2L1, Regulatory Protein SIR2 Homolog 1, hSIR2, hSIRT1, SIR2-Like Protein 1, SIR2alpha, NAD-Dependent Deacetylase Sirtuin-1, NAD-Dependent Protein Deacetylase Sirtuin-1, Sir2-Like 1, Sirtuin Type 1, EC 3.5.1.-.
Appearance
Sterile Filtered clear solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
SIRT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 280 amino acids (254-495) and having a molecular mass of 31.6 kDa.
SIRT1 is fused to a 38 amino acid His-tag at N-terminus.
SIRT1 is fused to a 38 amino acid His-tag at N-terminus.
Product Specs
Introduction
Sirtuin-1 (SIRT1) is a member of the sirtuin protein family, which are homologs to the yeast Sir2 protein. Sirtuin family members share a conserved sirtuin core domain and are classified into 4 classes. While the precise functions of human sirtuins are still being elucidated, yeast sirtuin proteins are known to play a role in regulating epigenetic gene silencing and suppressing rDNA recombination. It has been suggested that human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
Description
Recombinant human SIRT1, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 254-495. It has a molecular mass of 31.6 kDa and includes a 38 amino acid His-tag fused to the N-terminus.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The SIRT1 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4 M Urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
Sirtuin 1, SIRT1, SIR2L1, Regulatory Protein SIR2 Homolog 1, hSIR2, hSIRT1, SIR2-Like Protein 1, SIR2alpha, NAD-Dependent Deacetylase Sirtuin-1, NAD-Dependent Protein Deacetylase Sirtuin-1, Sir2-Like 1, Sirtuin Type 1, EC 3.5.1.-.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMKK IIVLTGAGVS VSCGIPDFRS RDGIYARLAV DFPDLPDPQA MFDIEYFRKD PRPFFKFAKE IYPGQFQPSL CHKFIALSDK EGKLLRNYTQ NIDTLEQVAG IQRIIQCHGS FATASCLICK YKVDCEAVRG DIFNQVVPRC PRCPADEPLA IMKPEIVFFG ENLPEQFHRA MKYDKDEVDL LIVIGSSLKV RPVALIPSSI PHEVPQILIN REPLPHLHFD VELLGDCDVI INELCHRLGG.
Product Science Overview
Gene and Protein Structure
Functions and Mechanisms
SIRT1 plays a crucial role in various cellular processes, including:
- Genomic Stability and DNA Repair: SIRT1 is involved in maintaining genomic stability and facilitating DNA repair mechanisms .
- p53-mediated Apoptosis: SIRT1 deacetylates the p53 protein, which is a key regulator of apoptosis (programmed cell death). By deacetylating p53, SIRT1 can deactivate its apoptotic function .
- Adipogenesis: SIRT1 influences the differentiation of preadipocytes into adipocytes, thereby playing a role in fat storage and metabolism .
- Aging: SIRT1 has been implicated in the regulation of aging processes. It is believed to promote longevity by mimicking the effects of caloric restriction, a known factor in extending lifespan .
Recombinant SIRT1
Recombinant SIRT1 refers to the SIRT1 protein that has been produced using recombinant DNA technology. This involves inserting the SIRT1 gene into a suitable expression system, such as E. coli, to produce the protein in large quantities. Recombinant SIRT1 is often used in research to study its functions and potential therapeutic applications .
Activators and Inhibitors
Several compounds have been identified as activators or inhibitors of SIRT1:
- Activators: Resveratrol, a polyphenol found in red wine, has been shown to increase the expression and activity of SIRT1. Other plant-derived polyphenols also interact with SIRT1 .
- Inhibitors: Nicotinamide is a known inhibitor of SIRT1. It competes with NAD+ and prevents SIRT1 from exerting its deacetylase activity .
Research and Therapeutic Potential
SIRT1 is a target of significant interest in the field of biomedical research due to its involvement in critical cellular processes and its potential therapeutic applications. Research is ongoing to explore the role of SIRT1 in diseases such as cancer, neurodegenerative disorders, and metabolic conditions. The development of SIRT1 modulators holds promise for therapeutic interventions aimed at enhancing healthspan and treating age-related diseases .
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