SIRT3 Human
Sirtuin 3 Human Recombinant
Cat. No.
BT27972
Source
Escherichia Coli.
Synonyms
Sirtuin 3, SIR2-like protein 3, Sir2-like 3, NAD-dependent deacetylase sirtuin-3, mitochondrial.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SIRT3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (118-399a.a.) and having a molecular mass of 33.5kDa.
SIRT3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SIRT3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
SIRT3, a member of the Sirtuin protein family, plays a crucial role in aging, stress resistance, and metabolic regulation. Characterized by a Sirtuin core domain, SIRT3 exhibits NAD+-dependent deacetylase activity within mitochondria. Its overexpression elevates mitochondrial uncoupling protein 1 levels. Notably, SIRT3 protein levels are significantly elevated in specific breast cancer types.
Description
Produced in E. coli, our SIRT3 is a single, non-glycosylated polypeptide chain encompassing amino acids 118-399. With a molecular mass of 33.5 kDa, this protein is fused to a 21 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The SIRT3 protein solution (1mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, and 10% glycerol.
Purity
Purity exceeds 95% as determined by SDS-PAGE.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Minimize freeze-thaw cycles.
Synonyms
Sirtuin 3, SIR2-like protein 3, Sir2-like 3, NAD-dependent deacetylase sirtuin-3, mitochondrial.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSDKGKLSLQ DVAELIRARA CQRVVVMVGA GISTPSGIPD FRSPGSGLYS NLQQYDLPYP EAIFELPFFF
HNPKPFFTLA KELYPGNYKP NVTHYFLRLL HDKGLLLRLY TQNIDGLERV SGIPASKLVE AHGTFASATC TVCQRPFPGE DIRADVMADR
VPRCPVCTGV VKPDIVFFGE PLPQRFLLHV VDFPMADLLL ILGTSLEVEP FASLTEAVRS SVPRLLINRD LVGPLAWHPR SRDVAQLGDV
VHGVESLVEL LGWTEEMRDL VQRETGKLDG PDK.
HNPKPFFTLA KELYPGNYKP NVTHYFLRLL HDKGLLLRLY TQNIDGLERV SGIPASKLVE AHGTFASATC TVCQRPFPGE DIRADVMADR
VPRCPVCTGV VKPDIVFFGE PLPQRFLLHV VDFPMADLLL ILGTSLEVEP FASLTEAVRS SVPRLLINRD LVGPLAWHPR SRDVAQLGDV
VHGVESLVEL LGWTEEMRDL VQRETGKLDG PDK.
Product Science Overview
Structure and Localization
Function
Role in Metabolism and Aging
SIRT3 has emerged as an important regulator of mitochondrial metabolism. Overexpression of SIRT3 in cultured cells has been shown to increase respiration and decrease the production of reactive oxygen species (ROS) . This suggests that SIRT3 plays a protective role against oxidative stress, which is a key factor in aging and various diseases .
Recombinant SIRT3
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