SERTAD1 Human
SERTA Domain Containing 1 Human Recombinant
Cat. No.
BT25225
Source
Escherichia Coli.
Synonyms
SERTA domain-containing protein 1, CDK4-binding protein p34SEI1, SEI-1, Transcriptional regulator interacting with the PHD-bromodomain 1, TRIP-Br1, SERTAD1, SEI1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SERTAD1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 260 amino acids (1-236 a.a) and having a molecular mass of 27.3kDa (Molecular weight on SDS-PAGE will appear higher).
SERTAD1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SERTAD1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
SERTAD1, or SERTA domain-containing protein, plays a crucial role in transcriptional regulation. It interacts with E2F-responsive promoters, enhancing their activity when bound to E2F-1/DP-1 transcription factors. This protein also influences cell cycle progression by reducing the activity of cyclin D1/CDK4 complexes that are resistant to the inhibitory effects of p16(INK4a). Its functionality is further diversified through interactions with various PHD- and bromodomain-containing proteins, including TIF1, TRIM28/TIF1B, and p300/CBP. Additionally, SERTAD1 exhibits binding affinity for DP1 and interacts with CDK4, highlighting its multifaceted role in cellular processes.
Description
This product consists of the recombinant human SERTAD1 protein, expressed in E. coli and purified to a high degree. The protein is a single, non-glycosylated polypeptide chain comprising 260 amino acids (with residues 1-236 representing the SERTAD1 sequence). A 24 amino acid His-tag is present at the N-terminus to facilitate purification. The molecular weight of the protein is 27.3 kDa, although it may appear larger on SDS-PAGE due to the His-tag. Purification is achieved using proprietary chromatographic techniques, resulting in a highly pure protein product.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The SERTAD1 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For longer-term storage, it is recommended to freeze the product at -20°C. To ensure stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). It's important to avoid repeated freeze-thaw cycles to maintain the protein's integrity.
Purity
The purity of the SERTAD1 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
SERTA domain-containing protein 1, CDK4-binding protein p34SEI1, SEI-1, Transcriptional regulator interacting with the PHD-bromodomain 1, TRIP-Br1, SERTAD1, SEI1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMLSKGL KRKREEEEEK EPLAVDSWWL DPGHTAVAQA PPAVASSSLF DLSVLKLHHS LQQSEPDLRH LVLVVNTLRR IQASMAPAAA LPPVPSPPAA PSVADNLLAS SDAALSASMA SLLEDLSHIE GLSQAPQPLA DEGPPGRSIG GAAPSLGALD LLGPATGCLL DDGLEGLFED IDTSMYDNEL WAPASEGLKP GPEDGPGKEE APELDEAELD YLMDVLVGTQ ALERPPGPGR.
Product Science Overview
Gene and Protein Information
SERTAD1 is involved in the regulation of transcription and cell cycle progression. It acts as a coregulator at E2F-responsive promoters, integrating signals provided by PHD- and/or bromodomain-containing transcription factors . This protein stimulates the transcriptional activity of E2F1/TFDP1 and renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of CDKN2A/p16INK4A .
Biological Functions
The primary functions of SERTAD1 include:
- Positive Regulation of Transcription: SERTAD1 is predicted to be involved in the epigenetic maintenance of chromatin in a transcription-competent conformation .
- Cell Cycle Progression: It plays a crucial role in cell cycle progression by interacting with cyclin D1/CDK4 and overcoming the inhibitory effects of CDKN2A/p16INK4A .
- Signal Integration: SERTAD1 integrates signals from PHD- and bromodomain-containing transcription factors, which are essential for various cellular processes .
Clinical Relevance
Recombinant SERTAD1
Recombinant human SERTAD1 protein, often tagged with a His-tag for purification purposes, is used in various research applications. It is typically expressed in E. coli and purified using conventional chromatography techniques . The recombinant protein is used to study the functions and interactions of SERTAD1 in a controlled environment.
Research Applications
The recombinant SERTAD1 protein is valuable in research for:
- Studying Protein-Protein Interactions: Understanding how SERTAD1 interacts with other proteins, such as cyclin D1/CDK4 and transcription factors .
- Investigating Transcriptional Regulation: Exploring the role of SERTAD1 in the regulation of gene expression and its impact on cellular processes .
- Disease Modeling: Using recombinant SERTAD1 to model diseases associated with its dysregulation, such as certain types of cancer and genetic disorders .
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