SCGB1A1 Rat
Uteroglobin Rat Recombinant
Cat. No.
BT29971
Source
Escherichia Coli.
Synonyms
Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SCGB1A1 Rat Recombinant produced in E.Coli is a homodimeric non-glycosylated polypeptide chains consisting of two 77 amino acids and having a molecular mass of 17.0kDa.
Product Specs
Introduction
Uteroglobin, also known as SCGB1A1, is a protein belonging to the Secretoglobin family. This multifunctional protein exhibits anti-inflammatory and anti-tumorigenic properties by binding to small hydrophobic molecules such as phospholipids and prostaglandins. It plays a role in various biological processes, including inflammation reduction, phospholipase A2 inhibition, and the sequestration of hydrophobic ligands. SCGB1A1 is primarily produced by Clara cells, the main non-ciliated, non-mucous secretory cells found in lung bronchioles, and other epithelia that interact with the external environment. In addition to capturing pro-inflammatory mediators and carcinogens, Uteroglobin is involved in inhibiting cell migration and invasion, platelet aggregation, and T cell differentiation. Defects in the SCGB1A1 gene are linked to an increased susceptibility to asthma.
Description
Recombinant SCGB1A1 from Rat, produced in E. coli, is a non-glycosylated homodimeric polypeptide chain. It consists of two units, each containing 77 amino acids, resulting in a molecular weight of 17.0 kDa.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The SCGB1A1 protein was lyophilized from a concentrated solution in PBS at a pH of 7.4, which had been filtered through a 0.2 µm filter.
Solubility
To reconstitute the lyophilized SCGB1A1, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized SCGB1A1 remains stable at room temperature for up to 3 weeks; however, it is advisable to store it in a desiccated state below -18°C. After reconstitution, SCGB1A1 should be stored at 4°C for no longer than 2-7 days. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 97.0% using the following methods:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity
The biological activity is comparable to the standard, demonstrating full activity. The ED50, determined by the immobilized protein's capacity to facilitate the adhesion of A549 human lung carcinoma cells, is less than 5.0 µg/ml. This corresponds to a specific activity greater than 200 IU/mg.
Synonyms
Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.
Source
Escherichia Coli.
Amino Acid Sequence
SSDICPGFLQ VLEALLLGSE SNYEAALKPF NPASDLQNAG TQLKRLVDTL PQETRINIVK LTEKILTSPL CEQDLRV
Product Science Overview
Discovery and Nomenclature
Expression and Tissue Distribution
Biological Functions
Uteroglobin plays several crucial roles in the body:
- Anti-inflammatory and Immunomodulatory Properties: It helps modulate the immune response and reduce inflammation .
- Phospholipid Binding: Uteroglobin binds to phospholipids, which are essential components of cell membranes .
- Regulation of Immune Response: It has been shown to regulate the activity of immune cells, contributing to its immunomodulatory effects .
Recombinant Uteroglobin
Recombinant uteroglobin is produced using genetic engineering techniques, where the gene encoding uteroglobin is inserted into a host organism, such as E. coli, to produce the protein in large quantities . This recombinant form retains the biological activity of the native protein and is used in various research and therapeutic applications.
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