SCGB1A1 Mouse
Uteroglobin Mouse Recombinant
Cat. No.
BT29908
Source
Escherichia Coli.
Synonyms
Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Uteroglobin Mouse Recombinant produced in E.coli is a non-glycosylated disulfide-linked homodimeric protein containing 2x75 amino acids chains and having a molecular mass of 16.7kDa.
The SCGB1A1 is purified by proprietary chromatographic techniques.
The SCGB1A1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Uteroglobin (SCGB1A1), a member of the Secretoglobin (SCGBs) family, is a versatile protein known for its anti-inflammatory and anti-tumorigenic properties. It achieves this by binding to small hydrophobic molecules, such as phospholipids and prostaglandins. Its functions include inflammation reduction, phospholipase A2 inhibition, and hydrophobic ligand sequestration. Primarily expressed by Clara cells (non-ciliated, non-mucous secretory cells) in lung bronchioles and other epithelia interacting with the external environment, SCGB1A1 sequesters pro-inflammatory mediators and carcinogens. Additionally, it plays a role in inhibiting cell migration and invasion, platelet aggregation, and T cell differentiation. Notably, defects in the SCGB1A1 gene are linked to an increased susceptibility to asthma.
Description
Recombinant Mouse Uteroglobin, produced in E. coli, is a non-glycosylated, disulfide-linked homodimeric protein. It consists of two amino acid chains, each containing 75 amino acids, resulting in a molecular mass of 16.7 kDa. The purification of SCGB1A1 is achieved using specialized chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The Uteroglobin protein solution, concentrated and filtered through a 0.2µm filter in PBS at pH 7.4, undergoes lyophilization to obtain the final product.
Solubility
To reconstitute the lyophilized SCGB1A1, it is recommended to dissolve it in sterile 18M-cm H₂O at a concentration of at least 100µg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
Lyophilized Uteroglobin remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it desiccated at temperatures below -18°C. Upon reconstitution, SCGB1A1 should be stored at 4°C for a period of 2 to 7 days. For long-term storage, adding a carrier protein like 0.1% HSA or BSA is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product exceeds 98.0%, as determined by the following methods:
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED₅₀, determined by the ability of the immobilized protein to support A549 human lung carcinoma cell adhesion, is less than 5.0µg/ml. This corresponds to a specific activity greater than 200 IU/mg.
Synonyms
Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.
Source
Escherichia Coli.
Amino Acid Sequence
DICPGFLQVL EALLMESESG YVASLKPFNP GSDLQNAGTQ LKRLVDTLPQ ETRINIMKLT EKILTSPLCK QDLRF.
Product Science Overview
Structure and Expression
Uteroglobin is a small, secreted, disulfide-bridged dimeric protein. It is composed of two 75 amino acid polypeptide chains, forming a homodimeric structure . The recombinant form of mouse uteroglobin is typically expressed in E. coli or HEK293 cells, and it is often tagged with a polyhistidine tag for purification purposes .
Biological Functions
Uteroglobin has several important biological functions:
- Anti-inflammatory and Immunomodulatory Effects: It inhibits soluble phospholipase A2 activity and binds to hydrophobic ligands such as phospholipids, prostaglandins, and polychlorinated biphenyls . This binding capability allows uteroglobin to modulate inflammatory responses and immune functions.
- Tumorigenic Inhibition: Uteroglobin has been shown to exert anti-tumorigenic effects by binding to small hydrophobic molecules .
Applications
Recombinant mouse uteroglobin is used in various research applications, including:
Storage and Stability
Recombinant mouse uteroglobin is typically lyophilized and stored at -20°C to -80°C for long-term stability. Upon reconstitution, it is most stable at -20°C to -80°C and can be stored for up to one week at 2-8°C . It is important to avoid repeated freeze-thaw cycles to maintain its stability and activity.
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