SARS Spike (14-667)
HEK293
Protein is >90% pure as determined SDS-PAGE.
Description
The HEK293 derived recombinant protein contains the SARS Coronavirus Spike S1 Gycoprotein, amino acids 14-667 fused to His tag at C-terminal.
Product Specs
HEK293
Purified by immobilized metal affinity chromatographic technique.
Product Science Overview
The SARS Spike (14-667 a.a.), Recombinant, refers to a specific segment of the spike (S) glycoprotein from the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV). This recombinant protein is expressed in human 293 cells (HEK293) and contains amino acids from Serine 14 to Arginine 667 . The spike protein is crucial for the virus’s ability to infect host cells and is a major target for vaccine development and therapeutic interventions.
The spike (S) glycoprotein of coronaviruses, including SARS-CoV, is essential for the virus’s ability to bind to host cells and initiate infection. The spike protein is composed of two subunits:
- S1 Subunit: Contains the receptor-binding domain (RBD) that engages with the host cell receptor, angiotensin-converting enzyme 2 (ACE2).
- S2 Subunit: Mediates the fusion between the viral and host cell membranes, facilitating viral entry into the host cell .
The recombinant SARS Spike (14-667 a.a.) includes the S1 subunit and part of the S2 subunit, making it a valuable tool for studying the virus’s entry mechanism and for developing neutralizing antibodies.
The recombinant SARS Spike (14-667 a.a.) is typically expressed in HEK293 cells, which are human embryonic kidney cells commonly used for protein production. The protein is purified and often tagged with a His-tag to facilitate purification and detection . The molecular weight of this recombinant protein is approximately 67.2 kDa .
The recombinant SARS Spike (14-667 a.a.) has several important applications:
- Vaccine Development: As a major immunogen, the spike protein is a key target for vaccine development. It induces neutralizing antibodies and T-cell responses, providing protective immunity against SARS-CoV .
- Therapeutic Research: The spike protein is a target for entry inhibitors, which can block the virus from entering host cells and prevent infection .
- Diagnostic Tools: The recombinant protein can be used in immunoassays to detect antibodies against SARS-CoV in patient samples, aiding in the diagnosis of SARS-CoV infections .
