SARS Spike (1-666)
HEK293
Protein is >85% pure as determined SDS-PAGE.
Description
The HEK293 derived recombinant protein contains the SARS Coronavirus Spike S1 Gycoprotein, amino acids 1-666 fused to His tag at C-terminal.
Product Specs
The SARS Coronavirus, an enveloped virus, possesses three key structural proteins on its surface: membrane (M), envelope (E), and spike (S) proteins. The spike (S) glycoprotein is particularly crucial as it facilitates viral entry into host cells by binding to a cellular receptor and mediating membrane fusion. Due to its pivotal role in the virus infection cycle, the S-protein is a prime target for neutralizing antibodies.
This recombinant protein, derived from HEK293 cells, consists of the SARS Coronavirus Spike S1 Glycoprotein (amino acids 1-666) with a C-terminal His tag.
The SARS CoV-Spike S1 protein solution is provided in DPBS (Dulbecco's Phosphate-Buffered Saline).
SARS CoV Spike S1 is shipped with ice packs to maintain its temperature. Upon receipt, store the protein at -20°C.
The purity of the protein is greater than 85% as determined by SDS-PAGE analysis.
HEK293
Purified by immobilized metal affinity chromatographic technique.
Product Science Overview
The Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) is a member of the betacoronavirus genus, which also includes the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) and the novel SARS-CoV-2 responsible for the COVID-19 pandemic. The spike (S) glycoprotein of SARS-CoV plays a crucial role in the virus’s ability to infect host cells. The recombinant SARS Spike (1-666 a.a.) protein is a truncated version of the full-length spike protein, encompassing the first 666 amino acids.
The spike protein is a transmembrane protein that forms homotrimers protruding from the viral surface. It is composed of two subunits, S1 and S2. The S1 subunit contains the receptor-binding domain (RBD), which is responsible for binding to the host cell receptor, angiotensin-converting enzyme 2 (ACE2). The S2 subunit mediates the fusion of the viral and host cell membranes, facilitating viral entry into the host cell.
The recombinant SARS Spike (1-666 a.a.) protein includes the entire S1 subunit and part of the S2 subunit. This region is critical for studying the virus’s interaction with the host cell and for developing vaccines and therapeutic antibodies.
Recombinant proteins are produced using various expression systems, such as bacteria, yeast, insect cells, and mammalian cells. The choice of expression system depends on the protein’s complexity and the need for post-translational modifications. For the SARS Spike (1-666 a.a.) protein, insect cell expression systems are commonly used due to their ability to produce properly folded and glycosylated proteins.
The recombinant SARS Spike (1-666 a.a.) protein is typically produced by cloning the gene encoding the spike protein into an expression vector, which is then introduced into the host cells. The host cells express the protein, which is subsequently purified using chromatography techniques.
The recombinant SARS Spike (1-666 a.a.) protein has several applications in research and medicine:
- Vaccine Development: The spike protein is a primary target for vaccine development because it elicits a strong immune response. Recombinant spike proteins are used to develop subunit vaccines, which contain only the antigenic parts of the virus.
- Antibody Production: The recombinant protein is used to generate monoclonal antibodies that can neutralize the virus. These antibodies are valuable for both therapeutic purposes and diagnostic assays.
- Structural Studies: Understanding the structure of the spike protein is essential for elucidating the mechanisms of viral entry and for designing antiviral drugs. Recombinant proteins are used in crystallography and cryo-electron microscopy studies to determine the protein’s structure.
- Diagnostic Assays: The recombinant spike protein is used in serological assays to detect antibodies in the blood of individuals who have been exposed to the virus. These assays are crucial for epidemiological studies and for assessing vaccine efficacy.
