SARS Spike Mosaic
Protein is >90% pure as determined SDS-PAGE.
Description
The E.coli derived recombinant protein contains the Spike Mosaic protein immunodominant regions 20-210 a.a. fused to 6xHis tag at C-terminal.
Product Specs
Severe acute respiratory syndrome (SARS) is a serious form of pneumonia caused by a coronavirus. Coronaviruses are a large family of viruses that can infect various animals, including humans, pigs, cows, mice, and cats. They typically cause mild respiratory illnesses. However, the origin of the coronavirus responsible for SARS remains unknown.
This recombinant protein, derived from E. coli, encompasses amino acids 20-210 of the SARS Spike protein's immunodominant region. It is fused with a 6xHis tag at its C-terminus.
The SARS Spike Mosaic protein is provided in a solution of 1xPBS with a pH of 7.8.
The protein is shipped with ice packs to maintain a low temperature. Upon receipt, it should be stored at -20°C.
SDS-PAGE analysis indicates that the protein purity exceeds 90%.
Purified by proprietary chromatographic technique.
Immunoreactive with sera of SARS Associated Coronavirus infected individuals.
RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TQDLFLPFFS NVTWFHAIHV SGTNGTKRFD
NPVLPFNDGV YFASTEKSNI IRGWIFGTTL DSKTQSLLIV NNATNVVIKV CEFQFCNDPF
LGVYYHKNNK SWMESEFRVY SSANNCTFEY VSQPFLMDLE GKQGNFKNLR EFVFKNIDGY
FKIYSKHTPI
Product Science Overview
The SARS-Associated Coronavirus Spike Mosaic, Recombinant, is a significant focus in virology and immunology research. This recombinant protein is derived from the spike (S) protein of SARS-related coronaviruses, which are known for their role in viral entry into host cells. The spike protein is a key target for vaccine development and therapeutic interventions due to its critical function in mediating the virus’s attachment and fusion with host cells.
The spike protein of SARS-related coronaviruses, including SARS-CoV and SARS-CoV-2, is a trimeric glycoprotein that protrudes from the viral surface. It consists of two subunits: S1 and S2. The S1 subunit contains the receptor-binding domain (RBD), which is responsible for binding to the host cell receptor, angiotensin-converting enzyme 2 (ACE2). The S2 subunit facilitates the fusion of the viral and host cell membranes, allowing the viral genome to enter the host cell .
Recombinant spike proteins are produced using genetic engineering techniques. These proteins are expressed in various host systems, such as mammalian cells, insect cells, or yeast, to ensure proper folding and post-translational modifications. The recombinant spike protein can be used in various applications, including vaccine development, serological assays, and structural studies .
- Vaccine Development: The spike protein is the primary target for COVID-19 vaccines. Recombinant spike proteins are used to elicit an immune response in the host, leading to the production of neutralizing antibodies that can prevent viral infection.
- Serological Assays: Recombinant spike proteins are used in diagnostic tests to detect antibodies against SARS-CoV-2 in patient samples. These assays help determine past infection and immunity status.
- Structural Studies: High-resolution structural studies of the spike protein, such as cryo-electron microscopy, provide insights into its conformational changes and interactions with host receptors. These studies are crucial for understanding viral entry mechanisms and designing antiviral drugs .
Recent studies have focused on the structural variations and mutational landscape of the spike protein. For instance, variations in structural motifs within SARS-related coronavirus spike proteins have been analyzed to understand their impact on viral pathogenicity and stability . Additionally, the mutational landscape of the spike receptor-binding domain has been mapped to identify key molecular determinants for virus-host interactions .
