SAA Feline
Greater than 95.0% as determined by SDS-PAGE.
Description
SAA Feline produced in E.coli is a single, non-glycosylated polypeptide chain (1-111 a.a) containing 121 a.a and having a molecular mass of 13,838 Dalton. SAA s fused with a 10 amino acids His tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Serum Amyloid A (SAA) is a family of apolipoproteins associated with high-density lipoprotein (HDL) in plasma. These proteins are highly conserved across vertebrate species and play a crucial role in the acute-phase response to inflammation. The recombinant form of feline Serum Amyloid A (APO-SAA) is produced for research purposes to study its biological functions and potential therapeutic applications.
The recombinant feline Serum Amyloid A (APO-SAA) is typically produced in Escherichia coli (E. coli) expression systems. The protein is a single, non-glycosylated polypeptide chain consisting of 121 amino acids, with a molecular mass of approximately 13.8 kDa . It is often fused with a 10 amino acid His tag at the N-terminus to facilitate purification through chromatographic techniques .
SAA proteins are involved in several critical biological processes:
- Acute-Phase Response: During inflammation, infection, or injury, SAA levels can increase dramatically, sometimes by as much as 1000-fold . This rapid rise helps in recruiting immune cells to the site of injury and modulating their activities, including cytokine production and phagocytosis .
- Lipid Metabolism: SAA interacts with HDL particles, displacing apolipoprotein A1 and transforming HDL into a pro-inflammatory particle. This alteration can affect the cardioprotective functions of HDL and potentially contribute to atherosclerosis and cardiovascular diseases .
- Immune Modulation: SAA acts as a chemoattractant, recruiting immune cells to sites of inflammation and injury. It also influences the activities of these cells, thereby shaping the immune response .
Recombinant feline SAA is used extensively in laboratory research to:
- Study the molecular mechanisms underlying the acute-phase response.
- Investigate the role of SAA in lipid metabolism and cardiovascular diseases.
- Develop potential therapeutic interventions for inflammatory and autoimmune diseases.
The lyophilized form of recombinant feline SAA is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. It is recommended to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent freeze-thaw cycles .
