SAA Canine
Greater than 95.0% as determined by SDS-PAGE.
Description
SAA Canine produced in E.coli is a single, non-glycosylated polypeptide chain (1-111 a.a) containing 121 a.a and having a molecular mass of 13,766 Dalton. SAA s fused with a 10 amino acids affinity tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Serum Amyloid A (SAA) is an acute-phase protein that plays a crucial role in the body’s response to inflammation. Initially identified for its involvement in amyloidosis, SAA has since been recognized for its diverse functions in health and disease. The recombinant form of Canine Serum Amyloid A (APO-SAA) is a valuable tool for research and diagnostic purposes, particularly in veterinary medicine.
The recombinant Canine Serum Amyloid A (APO-SAA) is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain. It consists of 121 amino acids and has a molecular mass of approximately 13,766 Daltons . The recombinant protein is fused with a 10 amino acid affinity tag at the N-terminus to facilitate purification through proprietary chromatographic techniques .
SAA is a major player in the acute-phase response, a rapid inflammatory response to tissue injury, infection, or other stressors. It is primarily produced by hepatic cells and secreted into the bloodstream, where it associates with high-density lipoproteins (HDL). This association is essential for its roles in cholesterol transport, immune modulation, and tissue regeneration .
- Inflammation Regulation: SAA is crucial for regulating inflammation by modulating the activity of immune cells and cytokines. It helps in the recruitment of immune cells to the site of injury or infection and promotes the clearance of pathogens and damaged cells .
- Tissue Repair: SAA plays a role in tissue repair by promoting the proliferation and migration of cells involved in wound healing. It also aids in the remodeling of extracellular matrix components .
- Lipid Metabolism: By associating with HDL, SAA is involved in lipid metabolism, particularly in the transport and clearance of cholesterol. This function is vital for maintaining lipid homeostasis during the acute-phase response .
The recombinant form of Canine Serum Amyloid A (APO-SAA) is widely used in veterinary research and diagnostics. It serves as a biomarker for inflammation and tissue damage in dogs. The rapid and dramatic increase in SAA levels during acute inflammation makes it an ideal marker for evaluating disease severity and monitoring the progression or resolution of illness .
The lyophilized form of recombinant Canine Serum Amyloid A (APO-SAA) is stable at room temperature for up to three weeks. For long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, it is recommended to store the protein at 4°C for short-term use (2-7 days) and below -18°C for long-term use. To prevent degradation, it is advisable to add a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) and avoid freeze-thaw cycles .
