S100A6 Human
Description
S100A6 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 110 amino acids (1-90 a.a.) and having a molecular mass of 12.3kDa. The S100A6 is purified by proprietary chromatographic techniques.
Product Specs
MGSSHHHHHH SSGLVPRGSH MACPLDQAIG LLVAIFHKYS GREGDKHTLS KKELKELIQK ELTIGSKLQD AEIARLMEDL DRNKDQEVNF QEYVTFLGAL ALIYNEALKG.
Product Science Overview
S100 Calcium Binding Protein A6 (S100A6), also known as calcyclin, is a member of the S100 protein family. This family comprises more than 20 low-molecular-weight proteins characterized by their ability to bind calcium ions through EF-hand motifs . S100A6 was first identified and purified over 30 years ago and has since been the subject of extensive research due to its involvement in various cellular processes and diseases .
S100A6 contains two EF-hand calcium-binding motifs: one at the N-terminus (atypical EF-hand) and one at the C-terminus (typical EF-hand) . Each monomer of S100A6 can bind two calcium ions, which induces a conformational change that exposes hydrophobic regions, facilitating interactions with target proteins . This calcium-dependent conformational change is crucial for its role in calcium signal transduction .
S100A6 is expressed in a variety of tissues and cell types, including fibroblasts, epithelial cells, and certain types of cancer cells . Its expression is regulated by several transcription factors such as c-Myc, p53, NF-κB, and Nrf2 . Additionally, epigenetic modifications like histone acetylation and DNA methylation also influence its expression . S100A6 is predominantly localized in the cytoplasm but can also be found in the nucleus and associated with cell membranes in the presence of calcium .
S100A6 plays a multifaceted role in cellular processes:
- Cytoskeletal Dynamics: It interacts with proteins such as annexins and tropomyosin, influencing cytoskeletal organization and cell motility .
- Cell Proliferation and Differentiation: S100A6 is involved in regulating cell cycle progression and differentiation, making it significant in both normal physiology and cancer .
- Stress Response: It participates in cellular responses to stress, including oxidative stress and mechanical stress .
- Signal Transduction: By binding to various intracellular and extracellular targets, S100A6 modulates signaling pathways that are crucial for cell survival and function .
Altered expression of S100A6 has been observed in several pathological conditions, particularly in cancers . Its expression levels often correlate with the stage and progression of tumors, suggesting a potential role in tumorigenesis . S100A6 is also being investigated as a biomarker for differential diagnosis and prognosis evaluation in various diseases .
