RWDD4 Human

RWDD4 is a non-glycosylated polypeptide chain consisting of 211 amino acids, with a molecular mass of approximately 23.6 kDa . The recombinant form of this protein is typically produced in E. coli and is fused to a 23 amino acid tag for purification purposes .

The exact function of RWDD4 is not fully understood, but proteins containing the RWD domain are generally involved in protein-protein interactions and may play roles in signal transduction, cell cycle regulation, and other critical cellular processes. The RWD domain itself is named after three proteins where it was first identified: RING finger-containing proteins, WD-repeat-containing proteins, and DEAD-like helicases.

Recombinant RWDD4 is used in various research applications, including:

• Protein-protein interaction studies: Understanding how RWDD4 interacts with other proteins can provide insights into its role in cellular processes.
• Functional assays: Investigating the biological activity of RWDD4 and its impact on cell behavior.
• Structural studies: Analyzing the structure of RWDD4 to understand its function and interactions at the molecular level.

Recombinant RWDD4 is produced using bacterial expression systems, such as E. coli. The protein is then purified using affinity chromatography techniques, which exploit the fusion tag added to the protein. The final product is typically supplied in a buffer solution containing Tris-HCl, NaCl, glycerol, and DTT to maintain stability and activity .