RWDD1 Human

The RWDD1 gene is located on chromosome 6q22.1 . The protein encoded by this gene consists of 243 amino acids and has an N-terminal RWD domain . The RWD domain is thought to be involved in protein-protein interactions, although its exact function is not fully understood .

RWDD1 is expressed in various tissues, with high expression levels observed in the thymus of young mice . However, its expression decreases significantly in aging mice and in those subjected to oxidative stress . In humans, RWDD1 is ubiquitously expressed, with notable enrichment in the cytosol of urethral epithelium .

RWDD1 functions as a transcription factor that regulates the expression of target genes . It has been shown to interact with the androgen receptor (AR) and enhance AR-dependent transactivation . Unlike many AR coregulators, RWDD1’s enhancement of AR-dependent transactivation is independent of the formation of the activation function-2 (AF2) hydrophobic protein-binding pocket in AR . Additionally, RWDD1 also activates glucocorticoid receptor (GR)-dependent transactivation .

Recombinant human RWDD1 protein is produced using conventional chromatography techniques and is often tagged with a His-tag at the N-terminus for purification purposes . This recombinant protein is used in various research applications to study the function and interactions of RWDD1.

While the exact clinical implications of RWDD1 are still being explored, its role in transcription regulation and interaction with key receptors like AR and GR suggests potential involvement in various physiological and pathological processes .