TROVE2 Human, His Biotin
Escherichia Coli.
60 kDa SS-A/Ro ribonucleoprotein, 60 kDa ribonucleoprotein Ro, RoRNP, 60 kDa Ro protein, Ro 60 kDa autoantigen, TROVE domain family member 2, Sjoegren syndrome type A antigen, SS-A, Sjoegren syndrome antigen A2, TROVE2, RO60, SSA2, RO-60.
Greater than 89% as determined by Capillary Electrophoresis.
Description
TROVE2 Human Recombinant Biotin produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 56kDa.
The TROVE2 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
The Ro 60 kDa autoantigen, also known as SSA2, is an RNA-binding protein that plays a role in protecting small cytoplasmic RNA molecules called Y RNAs from degradation. In individuals with Systemic Lupus Erythematosus (SLE), the immune system often misidentifies SSA2 as a foreign entity and produces antibodies against it.
TROVE2 Human Recombinant Biotin is a single, non-glycosylated polypeptide chain with a molecular weight of 56kDa, produced in E. coli. It features a 6xHis tag at the N-terminus to facilitate purification, which is carried out using proprietary chromatographic methods.
The protein is supplied in a solution containing 20mM HEPES (pH 8.0), 20% Glycerol, and 150mM NaCl.
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Repeated freezing and thawing should be avoided to maintain product integrity.
The purity of the product is greater than 89%, as determined by Capillary Electrophoresis analysis.
60 kDa SS-A/Ro ribonucleoprotein, 60 kDa ribonucleoprotein Ro, RoRNP, 60 kDa Ro protein, Ro 60 kDa autoantigen, TROVE domain family member 2, Sjoegren syndrome type A antigen, SS-A, Sjoegren syndrome antigen A2, TROVE2, RO60, SSA2, RO-60.
Escherichia Coli.
Product Science Overview
TROVE Domain Family Member 2, also known as TROVE2, is a protein that plays a crucial role in RNA binding. It is part of the TROVE domain family, which includes proteins involved in the stabilization and binding of RNA molecules. The human recombinant version of TROVE2, tagged with His and biotin, is produced for research purposes.
The human recombinant TROVE2 is a single, non-glycosylated polypeptide chain with a molecular mass of approximately 56-60 kDa . It is produced in Escherichia coli (E. coli) and is expressed with a 6xHis tag at the N-terminus. This tag facilitates purification through chromatographic techniques. The biotinylation of the protein allows for easy detection and interaction studies.
TROVE2 is primarily involved in binding several small cytoplasmic RNA molecules known as Y RNAs . These RNA molecules are crucial for various cellular processes, including RNA stability and degradation prevention. TROVE2 stabilizes these RNAs, protecting them from degradation.
In patients with autoimmune diseases such as Systemic Lupus Erythematosus (SLE), antibodies often react with the normal cellular TROVE2 protein, indicating its significance in immune response .
The biotinylated, His-tagged human recombinant TROVE2 is widely used in laboratory research. It is utilized in studies focusing on RNA-protein interactions, autoimmune disease mechanisms, and the development of diagnostic tools. The biotinylation allows for easy detection using streptavidin-based systems, making it a valuable tool in various biochemical assays.
