Introduction
RELM-beta, or Resistin-Like Molecule-beta, belongs to a newly discovered family of secreted proteins characterized by a conserved cysteine-rich C-terminus. This family includes resistin (also known as FIZZ3), RELM-alpha (FIZZ1), RELM-beta (FIZZ2), and RELM-gamma. While humans only possess resistin and RELM-beta, all four members are present in rodents. RELM-beta is primarily produced as a homodimer by intestinal goblet cells and is abundant in stool. Interestingly, RELM-beta is absent in the stool of germ-free mice with sterile intestinal tracts until they are exposed to bacteria in a natural environment. A subset of colon carcinoma cell lines has been observed to secrete RELM-beta into the cell culture supernatant. Currently, the physiological function of RELM-beta remains unclear. However, studies in mice have shown that high doses of recombinant RELM-beta can induce hyperglycemic effects, including reduced glucose disposal and increased hepatic glucose production.
Description
Recombinant Human RELM-b, produced in E. coli, is a non-glycosylated, disulfide-linked homodimer. Each polypeptide chain consists of 89 amino acids, resulting in a total molecular mass of 19 kDa. The purification of RELM-b is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
RELM-b is lyophilized in a solution of 0.1% Trifluoroacetic Acid (TFA).
Solubility
To reconstitute lyophilized RELM-b, it is recommended to dissolve it in sterile 0.1% TFA at a minimum concentration of 100 µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized RELM-b remains stable at room temperature for up to 3 weeks; however, for long-term storage, it should be stored desiccated at a temperature below -18°C. Once reconstituted, Human Recombinant RELM-b should be stored at 4°C for 2-7 days. For extended storage, it is advisable to store it below -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of RELM-b is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
Resistin-like beta, RELM beta, Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2.
Amino Acid Sequence
MQCSLDSVMD KKIKDVLNSL EYSPSPISKK LSCASVKSQG RPSSCPAGMA VTGCACGYGC GSWDVQLETT CHCQCSVVDW TTARCCHLT.
