RELM b Human, His

RELM-Beta Human Recombinant, His Tag
Cat. No.
BT20481
Source
Escherichia Coli.
Synonyms
Resistin-like beta, RELM beta, Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RELM-beta Human Recombinant is a His-Tagged Fusion Protein which is 11 kDa protein containing 90 amino acid residues of the RELM-beta human and 12 additional amino acid residues - HisTag (underlined).

Product Specs

Introduction
RELM-beta (Resistin-Like Molecule-beta), a member of a secreted protein family, is characterized by a conserved cysteine-rich C-terminus. This family comprises resistin (FIZZ3), RELM-alfa (FIZZ1), RELM-beta (FIZZ2), and RELM-gamma. While humans possess resistin and RELM-beta, rodents express all four. Produced as a homodimer solely by intestinal goblet cells, RELM-beta is abundant in stool. Notably, germ-free mice lack RELM-beta in their stool until bacterial colonization occurs upon exposure to a natural environment. Certain colon carcinoma cell lines secrete RELM-beta into the culture medium. Although the physiological function of RELM-beta remains unclear, high doses in mice have shown hyperglycemic effects, including reduced glucose disposal and elevated hepatic glucose production.
Description
RELM-beta Human Recombinant, a 11 kDa His-Tagged Fusion Protein, comprises 90 amino acids of human RELM-beta and a 12 amino acid HisTag (underlined).
Physical Appearance
White lyophilized powder, filtered.
Formulation
Lyophilized from a 0.5 mg/ml solution in 0.05M Acetate buffer, pH 4, after filtration.
Solubility
Dissolve the lyophilized pellet completely in 0.1M Acetate buffer, pH 4. For higher pH values, dilute the solution extensively with the appropriate buffer to a concentration of 10 µg/ml. The solubility of this antigen is limited at higher concentrations.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freeze-thaw cycles. The reconstituted protein remains stable at 4°C for a limited period and shows no change after two weeks at 4°C.
Purity
SDS-PAGE analysis indicates a purity greater than 95%.
Synonyms
Resistin-like beta, RELM beta, Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSTQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH.

Product Science Overview

Introduction

Resistin-like molecule-beta (RELM-β) is a member of the resistin-like molecule (RELM) family, which includes highly cysteine-rich proteins such as RELMα, RELMβ, Resistin, and RELMγ . These proteins are involved in various physiological and pathological processes, including inflammation, cell proliferation, glucose metabolism, and barrier defense .

Structure

RELM-β is a non-glycosylated, disulfide-linked homodimer. Each monomer contains 89 amino acids, with the dimer having a total molecular weight of 19 kDa . The human recombinant version of RELM-β is produced in E. coli and includes a His-tag for purification purposes . The His-tag consists of 12 additional amino acid residues, making it easier to isolate and purify the protein using affinity chromatography .

Function

RELM-β plays a significant role in various biological processes. It is involved in the inflammatory response, cell proliferation, glucose metabolism, and barrier defense . RELM-β has been shown to recruit CD4+ T cells during infectious colitis, promoting protective intestinal epithelial cell proliferation . It also exhibits antimicrobial activity against Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA) .

Applications

Recombinant human RELM-β is used primarily for research purposes. It is employed in studies investigating its role in inflammation, glucose metabolism, and barrier defense . The protein is also used to explore its potential as a therapeutic target for various diseases, including lung diseases, intestinal diseases, cardiovascular diseases, and cancers .

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