REG3A Human

Regenerating Islet-Derived 3 Alpha Human Recombinant
Cat. No.
BT11872
Source
Escherichia Coli.
Synonyms
Regenerating islet-derived protein 3 alpha, Reg III-alpha, Pancreatitis-associated protein 1, REG3A, HIP, PAP, PAP1, REG3, INGAP, PAP-H, PBCGF, REG-III.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The Recombinant Human REG3A protein is produced with N-terminal fusion of His Tag. The REG3A His -Tagged Fusion Protein is an 18.4 kDa protein containing 149 amino acid residues of the Human REG3A and 16 additional amino acid residues- His Tag (underlined).

Product Specs

Introduction
Pancreatitis-associated protein (PAP), also known as REG3A, is a protein primarily secreted by the pancreas during acute pancreatitis. Though its exact function is not fully understood, it's believed to have anti-bacterial and anti-apoptotic properties. Studies suggest it plays a role in modulating inflammation during pancreatitis and may contribute to lung inflammation by influencing TNF-alpha levels. PAP is also found in the liver, particularly during regeneration, and its presence in liver cancer suggests a potential role in tumor growth. In pancreatic cancer, PAP overexpression is common and correlates with tumor stage. Moreover, PAP is elevated in inflammatory bowel diseases, particularly Crohn's disease, and can help differentiate between ileal and colonic involvement.
Description
This product consists of the human REG3A protein with a His tag attached to its N-terminal end. It has a molecular weight of 18.4 kDa and is composed of 149 amino acids from the REG3A protein and 16 amino acids from the His tag.
Physical Appearance
White powder, lyophilized and filtered.
Formulation
Lyophilized from a 0.5 mg/ml solution in 0.05M Acetate buffer with a pH of 4. The solution was filtered through a 0.4 µm filter before lyophilization.
Purity
Determined by SDS-PAGE analysis to be greater than 95% pure.
Solubility
To create a working solution of around 0.5 mg/ml, add 0.1M Acetate buffer (pH 4) to the lyophilized powder and ensure complete dissolution. For solutions with higher pH, dilute the stock solution to 10 µg/ml using the appropriate buffer. This antigen has limited solubility at higher concentrations. Note: This product is not sterile. Filter it through a sterile filter before using in cell culture.
Stability
The lyophilized protein should be stored at -20°C. After reconstitution, aliquot the protein to minimize freeze-thaw cycles. The reconstituted protein can be stored at 4°C for a limited period (stable for up to two weeks).
Applications
Suitable for use in Western blotting and ELISA.
Synonyms
Regenerating islet-derived protein 3 alpha, Reg III-alpha, Pancreatitis-associated protein 1, REG3A, HIP, PAP, PAP1, REG3, INGAP, PAP-H, PBCGF, REG-III.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASHMEEPQ RELPSARIRC PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCNVRLPYV CKFTD.

Product Science Overview

Discovery and Structure

The Reg family was first identified in 1979 when pancreatic stone protein was observed in pancreatic stones and juice obtained from patients with chronic calcifying pancreatitis . The protein was later named “regenerating gene” due to its increased expression in regenerating and hyperplastic islets . REG3A is a 19-kilodalton secreted calcium-dependent lectin protein, which means it binds to carbohydrates in a calcium-dependent manner .

Expression and Regulation

REG3A is primarily expressed in the digestive system, including the pancreas, liver, and intestines . Its expression is regulated by various factors such as injury, infection, inflammatory stimuli, and pro-cytokines . During pancreatic inflammation and liver carcinogenesis, the expression of REG3A is significantly enhanced .

Functions

REG3A has multiple roles in the human body:

  1. Tissue Repair: It acts as a tissue-repair molecule, promoting the regeneration of damaged tissues .
  2. Antimicrobial Defense: REG3A has bactericidal properties, helping to protect the body against bacterial infections .
  3. Anti-inflammatory: It plays a role in reducing inflammation by modulating the immune response .
  4. Cell Proliferation and Apoptosis: REG3A is involved in cell proliferation and apoptosis, which are essential processes for maintaining tissue homeostasis .
Clinical Significance

REG3A has been identified as a prognostic biomarker for gastrointestinal chronic graft-versus-host disease (GI-cGVHD), a condition that occurs in patients who have undergone allogeneic hematopoietic cell transplantation . Plasma levels of REG3A are significantly increased in patients with GI-cGVHD compared to those without the condition . High levels of REG3A are associated with a higher likelihood of non-relapse mortality in these patients .

Additionally, REG3A has been found to be overexpressed in various gastrointestinal cancers, including hepatocellular carcinoma, pancreatic cancer, gastric cancer, and colorectal cancer . Its role in cancer is complex, with studies showing both oncogenic and tumor-suppressive effects depending on the context .

Potential Therapeutic Applications

Given its involvement in tissue repair, antimicrobial defense, and inflammation, REG3A holds potential as a therapeutic target for various diseases. Understanding its regulation and function could lead to the development of new treatments for conditions such as chronic inflammatory diseases, infections, and cancer .

In conclusion, Regenerating Islet-Derived 3 Alpha (Human Recombinant) is a multifunctional protein with significant roles in tissue repair, antimicrobial defense, and inflammation. Its clinical significance as a biomarker and potential therapeutic target makes it an important molecule in medical research and treatment.

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