REG3A Antibody
Description
Product Specs
MRGSHHHHHH GMASHMEEPQ RELPSARIRC PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCNVRLPYV CKFTD.
Product Science Overview
Regenerating islet-derived 3 alpha (REG3A) is a protein that plays a crucial role in the body’s immune response and tissue regeneration. It is part of the Regenerating gene (Reg) family, which is known for its involvement in cell proliferation, differentiation, and survival. The polyclonal rabbit anti-human REG3A antibody is a valuable tool in biomedical research, particularly in studies related to pancreatic function, inflammation, and cancer.
REG3A, also known as hepatointestinal pancreatic protein (HIP/PAP), is a member of the C-type lectin family. It is primarily expressed in the pancreas and small intestine, where it is involved in the regeneration of islet cells and the maintenance of intestinal homeostasis. REG3A has antimicrobial properties and is known to bind to bacterial peptidoglycans, thereby playing a role in the innate immune response .
Polyclonal antibodies are produced by immunizing animals, such as rabbits, with an antigen—in this case, the human REG3A protein. The immune system of the rabbit generates a diverse array of antibodies that recognize multiple epitopes on the antigen. This polyclonal nature makes these antibodies highly sensitive and capable of detecting the target protein in various applications, including immunohistochemistry (IHC), immunofluorescence (IF), and western blotting .
The production of polyclonal rabbit anti-human REG3A antibodies involves several steps:
- Immunization: Rabbits are immunized with a recombinant fragment of the human REG3A protein. This fragment typically corresponds to a specific region of the protein, such as amino acids 100 to the C-terminus .
- Antibody Collection: After a series of booster immunizations, blood is collected from the rabbits, and the serum is separated. This serum contains the polyclonal antibodies.
- Purification: The antibodies are purified using affinity chromatography, where the target antigen is used as a ligand to selectively capture the specific antibodies from the serum .
Polyclonal rabbit anti-human REG3A antibodies are widely used in research to study the expression and function of REG3A in various biological contexts:
- Immunohistochemistry (IHC): These antibodies are used to detect REG3A in tissue sections, providing insights into its localization and abundance in different tissues .
- Immunofluorescence (IF): By labeling the antibodies with fluorescent dyes, researchers can visualize the distribution of REG3A in cells and tissues under a fluorescence microscope .
- Western Blotting: These antibodies are used to detect REG3A in protein extracts, allowing researchers to quantify its expression levels in different samples .
REG3A has been implicated in various physiological and pathological processes, making it a significant target for research:
- Pancreatic Function: REG3A is involved in the regeneration of pancreatic islet cells, which are crucial for insulin production and glucose homeostasis .
- Inflammation: REG3A plays a role in the immune response to bacterial infections, particularly in the gut, where it helps maintain intestinal barrier integrity .
- Cancer: Altered expression of REG3A has been observed in several cancers, including pancreatic and colorectal cancers, suggesting its potential as a biomarker for diagnosis and prognosis .
