RBP4 Antibody

Retinol Binding Protein-4, Mouse Anti Human
Cat. No.
BT9713
Source
Synonyms
Retinol Binding Protein 4, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP.
Appearance
Purity
Usage
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Description

Product Specs

Introduction
Retinol binding protein 4 (RBP4) is a member of the lipocalin protein family and serves as the specific carrier for retinol (vitamin A alcohol) in the bloodstream. This protein, primarily produced and secreted by adipose tissue, has been strongly linked to insulin resistance. Its primary function is to transport retinol from storage sites in the liver to peripheral tissues. Within the plasma, RBP4 forms a complex with retinol, which then interacts with transthyretin. This interaction prevents the complex from being filtered out by the kidneys and excreted in urine. Essentially, RBP4 acts as a delivery system for retinol, ensuring its safe passage from the liver to various tissues in the body, while transthyretin plays a crucial role in maintaining retinol levels in circulation.
Formulation
The antibody is supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) at a pH of 7.4. The formulation also contains 0.1% sodium azide as a preservative.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be kept at 4°C. For long-term storage, it is recommended to store the antibody at -20°C. Repeated freezing and thawing cycles should be avoided to maintain antibody stability and activity.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored properly at -20°C. At 4°C, the antibody can be stored for up to 1 month.
Applications
The RBP4 antibody has been rigorously tested in both ELISA and Western blot applications to ensure its specificity and reactivity. However, optimal working dilutions may vary depending on the specific application and experimental conditions. For Western blot analysis, a dilution range of 1:500 to 1:1000 is recommended, with an initial starting dilution of 1:500 suggested. Users are encouraged to perform titration experiments to determine the optimal antibody dilution for their specific application.
Synonyms
Retinol Binding Protein 4, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP.
Purification Method
RBP4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT2B4AT.
Immunogen
Anti-human RBP4 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human RBP4 amino acids 19-201 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Introduction

Retinol Binding Protein-4 (RBP4) is a member of the lipocalin family and serves as the specific carrier for retinol (vitamin A alcohol) in the blood. It plays a crucial role in the transport of retinol from the liver to peripheral tissues. The mouse anti-human RBP4 antibody is a monoclonal antibody that specifically detects human RBP4 in various applications such as Western blotting and flow cytometry.

Structure and Function

RBP4 is a small protein with a molecular weight of approximately 21 kDa. It binds retinol and forms a complex with transthyretin (TTR) in the plasma. This complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex . The primary function of RBP4 is to deliver retinol from liver stores to peripheral tissues, where it can be utilized for various biological processes, including vision, immune function, and cellular growth .

Gene and Protein Information

The RBP4 gene is located on chromosome 10 in humans and encodes the retinol-binding protein. The gene is highly conserved across species, and its expression is regulated by retinoic acid levels. In conditions of vitamin A deficiency, the secretion of RBP4 is blocked, leading to impaired delivery of retinol to target tissues .

Clinical Significance

Alterations in RBP4 levels have been associated with various metabolic and inflammatory conditions. Elevated levels of RBP4 have been linked to insulin resistance, obesity, and type 2 diabetes. Conversely, low levels of RBP4 can result in vitamin A deficiency, affecting vision and immune function .

Applications of Mouse Anti-Human RBP4 Antibody

The mouse anti-human RBP4 antibody is widely used in research to study the expression and function of RBP4 in different tissues and under various physiological conditions. It is particularly useful in techniques such as Western blotting, where it can detect RBP4 in human and mouse samples . Additionally, it is used in flow cytometry to analyze the presence of RBP4 on the surface of cells .

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