RAP2A Human

RAP2A, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10617
Source
Escherichia Coli.
Synonyms
Ras-related protein Rap-2a, RbBP-30, RAP2A, KREV, RAP2, K-REV.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

RAP2A Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 200 amino acids (1-180 a.a.) and having a molecular mass of 22.4kDa. The RAP2A is purified by proprietary chromatographic techniques.

Product Specs

Introduction
RAP2A, a member of the RAS oncogene family, is a Ras-like small G protein. It shares 60% identity with Rap1A and possesses a carboxy-terminal CAAX motif and two upstream cysteines, similar to H-Ras, K-Ras, and N-Ras proteins. Unlike Rap1A and Rap1B, RAP2A overexpression doesn't hinder the Ras signaling pathway.
Description
RAP2A Human Recombinant is a single, non-glycosylated polypeptide chain with a 20 amino acid His tag at the N-terminus. Produced in E. coli, it contains 200 amino acids (1-180 a.a.) and has a molecular weight of 22.4kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The RAP2A solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 95.0% as assessed by SDS-PAGE.
Synonyms
Ras-related protein Rap-2a, RbBP-30, RAP2A, KREV, RAP2, K-REV.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI IRVKRYEKVP VILVGNKVDL ESEREVSSSE GRALAEEWGC PFMETSAKSK TMVDELFAEI VRQMNYAAQP DKDDPCCSAC.

Product Science Overview

Gene and Protein Information
  • Gene Symbol: RAP2A
  • Official Name: RAP2A, member of RAS oncogene family
  • Aliases: K-REV, Ras-Related Protein Rap-2a, RbBP-30, RAP2
  • Ensembl ID: ENSG00000125249
  • NCBI Gene ID: 5911
  • UniProtKB/Swiss-Prot ID: P10114
Function and Mechanism

RAP2A is a small GTP-binding protein that cycles between an inactive GDP-bound form and an active GTP-bound form . In its active form, RAP2A interacts with and regulates several effectors, including MAP4K4, MINK1, and TNIK . These interactions are crucial for various cellular processes, such as:

  • Actin Cytoskeleton Reorganization: RAP2A plays a role in the reorganization of the actin cytoskeleton, which is essential for cell shape, motility, and division .
  • Microvillus Assembly: It is involved in the assembly of microvilli, which are small, finger-like projections on the surface of epithelial cells that increase surface area for absorption .
  • Protein Autophosphorylation: RAP2A positively regulates protein autophosphorylation, a process where a protein phosphorylates itself to activate or deactivate its function .
Cellular Localization

RAP2A is located in the plasma membrane and recycling endosome membrane . This localization is important for its role in signaling pathways and cellular processes.

Associated Diseases

Mutations or dysregulation of RAP2A have been associated with several diseases, including:

  • Sporadic Breast Cancer: RAP2A has been implicated in the development of sporadic breast cancer .
  • Ullrich Congenital Muscular Dystrophy 1A: This gene is also associated with Ullrich congenital muscular dystrophy 1A, a genetic disorder characterized by muscle weakness and joint abnormalities .
Signaling Pathways

RAP2A is involved in multiple signaling pathways, including:

  • Activation of cAMP-Dependent PKA: This pathway is crucial for various cellular responses to hormonal stimulation .
  • ERK Signaling: RAP2A is part of the ERK signaling pathway, which is involved in cell division, differentiation, and survival .
Research and Clinical Relevance

Due to its involvement in critical cellular processes and diseases, RAP2A is a significant target for research. Understanding its function and regulation can provide insights into the development of therapeutic strategies for related diseases.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

RAP1A Human
RAP1A, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10454
Source
Escherichia Coli.
RAP1B Human
RAP1B, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10536
Source
Escherichia Coli.
RAP2B Human
RAP2B, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10716
Source
E.coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.