RAP1B Human

RAP1B, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10536
Source
Escherichia Coli.
Synonyms
RAP1B, member of RAS oncogene family, DKFZp586H0723, K-REV, RAL1B, GTP-binding protein smg p21B, Ras family small GTP binding protein RAP1B, RAS-related protein RAP1B, small GTP binding protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RAP1B produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181.a.a) and having a molecular mass of 22.6kDa.
RAP1B is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RAP1B, a member of the RAS-like small GTP-binding protein superfamily, plays a crucial role in cell signaling. This protein exhibits intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. Notably, the putative effector domain of Ras proteins, essential for cell transformation and interaction with the effector protein GAP, is conserved in both Rap1 proteins.
Description
Produced in E. coli, our recombinant RAP1B protein is a single, non-glycosylated polypeptide chain comprising 201 amino acids (1-181 a.a.). It has a molecular weight of 22.6 kDa. For purification purposes, a 20 amino acid His-tag is fused to the N-terminus of the protein, and purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The RAP1B protein solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 5 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the protein should be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of our RAP1B protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
RAP1B, member of RAS oncogene family, DKFZp586H0723, K-REV, RAL1B, GTP-binding protein smg p21B, Ras family small GTP binding protein RAP1B, RAS-related protein RAP1B, small GTP binding protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD
LVRQINRKTP VPGKARKKSS C.

Product Science Overview

Introduction

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. This family of proteins plays a crucial role in regulating various cellular processes, including cell adhesion, growth, and differentiation. RAP1B is encoded by the RAP1B gene and is known for its involvement in integrin-mediated cell signaling.

Gene and Protein Information

The RAP1B gene is located on chromosome 12 and encodes a protein that is part of the RAS oncogene family. The protein itself is a GTP-binding protein that possesses intrinsic GTPase activity. This activity is essential for the protein’s function in cellular signaling pathways .

Function and Mechanism

RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. Integrins are transmembrane receptors that facilitate cell-extracellular matrix (ECM) adhesion. RAP1B plays a role in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. It is required for the localization of phosphorylated PRKCZ, PARD3, and TIAM1 to the cell junction, which is crucial for the establishment of basal endothelial barrier function .

Biological Significance

RAP1B is involved in multiple cellular processes, including:

  • Cell Adhesion: By regulating integrin-mediated signaling, RAP1B influences how cells adhere to each other and to the ECM.
  • Cell Growth and Differentiation: As a member of the RAS-like small GTP-binding protein superfamily, RAP1B is involved in pathways that control cell growth and differentiation.
  • Endothelial Cell Polarity: RAP1B contributes to the polarizing activity of KRIT1 and CDH5, which is essential for maintaining endothelial cell polarity and vascular lumen .
Clinical Relevance

Mutations or dysregulation of RAP1B have been associated with various diseases. For instance, RAP1B is linked to Thrombocytopenia 11 with multiple congenital anomalies and dysmorphic facies, and Kabuki Syndrome 1. These associations highlight the importance of RAP1B in normal cellular function and development .

Research and Applications

Human recombinant RAP1B is used in various research applications to study its function and role in cellular processes. Recombinant proteins are produced through recombinant DNA technology, which allows for the expression of the protein in various host systems. This technology is crucial for studying the protein’s structure, function, and interactions in a controlled environment.

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THE BIOTEK
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