RAD51D (1-328) Human

RAD51D (1-328 a.a.) Human Recombinant
Cat. No.
BT10853
Source
Escherichia Coli.
Synonyms
BROVCA4, R51H3, RAD51L3, TRAD, DNA repair protein RAD51 homolog 4, RAD51 homolog D, RAD51-like protein 3, RAD51D.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RAD51D (1-328) Human Recombinant produced in E. coli is. a single polypeptide chain containing 351 amino acids and having a molecular mass of 37.4kDa. RAD51D (1-328) is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RAD51D belongs to the RAD51 protein family, which shares significant similarity with bacterial RecA and Saccharomyces cerevisiae Rad51. These proteins play a crucial role in DNA repair and homologous recombination. Specifically, RAD51D interacts with other RAD51 family members to form a complex that facilitates homologous pairing between single- and double-stranded DNA, contributing to the early stages of DNA recombinational repair.
Description
RAD51D (1-328) Human Recombinant is a protein produced in E. coli. It is a single polypeptide chain consisting of 351 amino acids, with a molecular weight of 37.4kDa. A 23 amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized through filtration.
Formulation
The RAD51D (1-328) solution is provided at a concentration of 0.25mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure long-term stability, adding a carrier protein like HSA or BSA (0.1%) is advisable. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the RAD51D (1-328) protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
BROVCA4, R51H3, RAD51L3, TRAD, DNA repair protein RAD51 homolog 4, RAD51 homolog D, RAD51-like protein 3, RAD51D.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KALVALRRVL LAQFSAFPVN GADLYEELKT STAILSTGIG SLDKLLDAGL YTGEVTEIVG GPGSGKTQVC LCMAANVAHG LQQNVLYVDS NGGLTASRLL QLLQAKTQDE EEQAEALRRI QVVHAFDIFQ MLDVLQELRG TVAQQVTGSS GTVKVVVVDS VTAVVSPLLG GQQREGLALM MQLARELKTL ARDLGMAVVV TNHITRDRDS GRLKPALGRS WSFVPSTRIL LDTIEGAGAS GGRRMACLAK SSRQPTGFQE MVDIGTWGTS EQSATLQGDQ T.

Product Science Overview

Structure and Expression

The RAD51D (1-328 a.a.) (Human Recombinant) protein is a human full-length protein expressed in Escherichia coli. It consists of 328 amino acids and has a molecular weight of approximately 37.4 kDa . The recombinant version of this protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection .

Function and Mechanism

RAD51D is involved in the HRR pathway, which is essential for repairing double-stranded DNA breaks. It binds to single-stranded DNA (ssDNA) and exhibits DNA-dependent ATPase activity. RAD51D is a part of the BCDX2 complex, which also includes RAD51B, RAD51C, and XRCC2. This complex acts in the BRCA1-BRCA2-dependent HR pathway, playing a role downstream of BRCA2 recruitment and upstream of RAD51 recruitment .

The BCDX2 complex predominantly binds to the intersection of the four duplex arms of the Holliday junction and the junction of replication forks. It has been reported to bind single-stranded DNA, single-stranded gaps in duplex DNA, and specifically to nicks in duplex DNA. Additionally, RAD51D is involved in telomere maintenance .

Clinical Significance

Mutations in the RAD51D gene are associated with a high risk of ovarian cancer. Studies have shown that mutations in RAD51D, along with BRIP1 and RAD51C, significantly increase the susceptibility to ovarian cancer. These mutations are considered high-risk factors for ovarian cancer, second only to BRCA1 and BRCA2 mutations .

Applications

The recombinant RAD51D (1-328 a.a.) protein is used in various research applications, including:

  • SDS-PAGE: To analyze the protein’s purity and molecular weight.
  • Mass Spectrometry (MS): For detailed protein characterization.
  • Functional Studies: To investigate the protein’s role in DNA repair and its interaction with other proteins in the HRR pathway .

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