RAD51B Human

RAD51B Human Recombinant
Cat. No.
BT10793
Source
Escherichia Coli.
Synonyms
RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2, DNA repair protein RAD51 homolog 2, Rad51B, RAD51-like protein 1, RAD51B.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RAD51B Human Recombinant produced in E. coli is. a single polypeptide chain containing 373 amino acids (1-350) and having a molecular mass of 40.6kDa. RAD51B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RAD51B belongs to the RAD51 protein family, which plays a crucial role in DNA repair via homologous recombination. This evolutionary conserved protein is essential for maintaining genomic stability. As a component of the homologous recombination repair (HRR) pathway, RAD51B contributes to the repair of double-stranded DNA breaks that may arise during DNA replication or due to DNA-damaging agents. It forms a stable heterodimer with RAD51C, another member of the RAD51 family. This complex interacts with other family members like RAD51, XRCC2, and XRCC3 to execute DNA repair. It's important to note that overexpression of RAD51B can induce cell cycle delay in the G1 phase and trigger apoptosis.
Description
Recombinant RAD51B protein, of human origin, has been expressed in E. coli. This single polypeptide chain consists of 373 amino acids (residues 1 to 350) and has a molecular weight of 40.6 kDa. For purification purposes, a 23 amino acid His-tag has been added to the N-terminus. Purification is carried out using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, sterile-filtered solution.
Formulation
The provided solution contains RAD51B at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. To maintain product integrity, avoid repeated freezing and thawing cycles.
Purity
Analysis by SDS-PAGE confirms that the purity of this protein is greater than 85%.
Synonyms
RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2, DNA repair protein RAD51 homolog 2, Rad51B, RAD51-like protein 1, RAD51B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS GSSCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI KEEGLVLQAY GNS.

Product Science Overview

Homologous Recombination and DNA Repair

Homologous recombination (HR) is a critical pathway for repairing DNA double-strand breaks (DSBs), which are among the most severe types of DNA damage. DSBs can be caused by various factors, including ionizing radiation, radiomimetic chemicals, and DNA replication inhibitors . The HR pathway ensures that the genetic information is accurately repaired by using a homologous sequence as a template, thereby preserving the genetic code.

RAD51 Family and Its Role

The RAD51 family includes several proteins, such as RAD51, RAD51B, RAD51C, RAD51D, XRCC2, XRCC3, and DMC1 . These proteins are homologous to the bacterial RecA protein and are involved in the formation of nucleoprotein filaments on single-stranded DNA. These filaments are essential for the search for homology and strand pairing during HR.

Specific Role of RAD51B

RAD51B, in particular, is involved in the early stages of HR. It forms a complex with other RAD51 paralogs, such as RAD51C and XRCC3, to facilitate the repair process . RAD51B is essential for the maintenance of genome integrity, resistance to DNA-damaging agents, and gene targeting . It has been shown to play a significant role in both somatic and meiotic recombination, ensuring proper chromosomal disjunction during meiosis .

Clinical Significance

Mutations and variations in the RAD51B gene have been associated with various cancers, including hereditary breast and ovarian cancer syndrome and prostate cancer . Additionally, enhancer variants of RAD51B have been linked to susceptibility and progression of glioma, a type of brain tumor . These variants can affect the expression of RAD51B and its ability to repair DNA, thereby influencing cancer risk and progression.

Research and Applications

Research on RAD51B continues to be of great interest due to its critical role in DNA repair and its implications in cancer biology. Understanding the mechanisms by which RAD51B and its paralogs function can lead to the development of targeted therapies for cancer treatment. Recombinant RAD51B protein is often used in research to study these mechanisms and to develop potential therapeutic interventions.

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