RAC1 Human, His

Ras-Related C3 Botulinum Toxin substrate 1 Human Recombinant, His Tag
Cat. No.
BT11246
Source
Escherichia Coli.
Synonyms
P21-RAC1, RAC-1, RAC1, RAS-like protein TC25, MIG5, Cell-migration-inducing gene 5 protein,Ras-related C3 botulinum toxin substrate 1, rho family small GTP binding protein Rac1, TC-25, MGC111543.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Ras-Related C3 Botulinum Toxin substrate 1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 212 amino acids (1-192 a.a.) and having a molecular mass of 23.6 kDa. RAC1 contains a 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.

Product Specs

Introduction
RAC1, a member of the RAS superfamily of small GTP-binding proteins, functions as a GTPase. This superfamily plays a crucial role in regulating various cellular processes, including cell growth, cytoskeletal reorganization, and protein kinase activation. Specifically, RAC1 regulates the actin cytoskeleton and participates in cell-cell adhesion regulation.
Description
Recombinant Human Ras-Related C3 Botulinum Toxin substrate 1, expressed in E. coli, is a non-glycosylated polypeptide chain. It consists of 212 amino acids (1-192 a.a.) with a 20 amino acid His Tag at the N-terminus, resulting in a molecular weight of 23.6 kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
The RAC1 His Tag protein is supplied in a solution containing 20mM Tris-HCl (pH 7.5), 1mM EDTA, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
P21-RAC1, RAC-1, RAC1, RAS-like protein TC25, MIG5, Cell-migration-inducing gene 5 protein,Ras-related C3 botulinum toxin substrate 1, rho family small GTP binding protein Rac1, TC-25, MGC111543.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPLSYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSALTQRGLKTVFD EAIRAVLCPP PVKKRKRKCL LL.

Product Science Overview

Introduction

Ras-related C3 botulinum toxin substrate 1, commonly known as Rac1, is a small (~21 kDa) signaling G protein and a member of the Rac subfamily of the Rho family of GTPases. It is encoded by the RAC1 gene in humans . Rac1 plays a crucial role in various cellular processes, including cell growth, cytoskeletal reorganization, and the activation of protein kinases .

Structure and Function

Rac1 is a pleiotropic regulator involved in many cellular processes such as the cell cycle, cell-cell adhesion, motility, and epithelial differentiation . It is particularly important in the formation of lamellipodia, which are essential for cell motility . Rac1 is ubiquitously expressed in tissues and is vital for maintaining epidermal stem cells .

Role in Disease

Rac1 has been implicated in several diseases, particularly cancer and diabetic nephropathy. In cancer, Rac1 is known to drive cell motility, which is a hallmark of cancer cell invasion and metastasis . Overexpression of Rac1 has been linked to tumor formation and metastasis . In diabetic nephropathy, Rac1 activation accelerates renal damage through the activation of the MLK3-MKK7-JNK signaling module .

Recombinant Rac1 (Human, His Tag)

Recombinant Rac1 proteins are often tagged with a His (histidine) tag to facilitate purification and detection. The His tag is a string of histidine residues that binds to nickel ions, allowing the protein to be easily isolated using nickel-affinity chromatography. This recombinant form is used in various research applications to study the protein’s function and its role in disease.

Research Applications

Recombinant Rac1 is widely used in biochemical and cellular studies to understand its role in signaling pathways. It is also used in drug discovery research to identify potential inhibitors that could modulate Rac1 activity and provide therapeutic benefits in diseases like cancer and diabetic nephropathy .

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