PSPN Mouse
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Product Specs
For reconstitution, it is recommended to dissolve the lyophilized PSPN in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. Further dilutions can be made in other aqueous solutions.
(a) RP-HPLC analysis.
(b) SDS-PAGE analysis.
Product Science Overview
Persephin shares 38-46% amino acid identity with other family members such as GDNF, neurturin, and artemin . The recombinant mouse persephin protein is typically produced in Escherichia coli (E. coli) and purified to high levels of purity, often exceeding 95% . The protein is a disulfide-linked homodimer with a predicted molecular mass of approximately 10.3 kDa per monomer .
Persephin has been shown to promote the survival and growth of central dopaminergic and motor neurons . It is also involved in kidney development, although it does not support the survival of peripheral neurons . The biological activity of recombinant persephin is often measured using cell proliferation assays, such as those involving human medullary thyroid cancer cells (TT cells). The effective dose (ED50) for this activity is typically in the range of 1-3 ng/mL .
Recombinant mouse persephin is used in various research applications, including studies on neuroprotection, neuroregeneration, and kidney development . It is available in both carrier-free and carrier-containing formulations. The carrier protein, often bovine serum albumin (BSA), is added to enhance protein stability and shelf-life .
The recombinant protein is usually lyophilized and should be reconstituted in a suitable buffer before use. It is recommended to store the protein at -20 to -70°C to maintain its stability. Once reconstituted, the protein can be stored at 2-8°C for up to one month or at -20 to -70°C for up to three months under sterile conditions .
