PSPN Human
Persephin Human Recombinant
Cat. No.
BT11396
Source
Escherichia Coli.
Synonyms
Persephin, PSP, PSPN.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PSPN Human Recombinant produced in E.Coli is a disulfide-linked homodimer containing 2x96 amino acids and having a molecular mass of 20.5kDa.
The PSPN is purified by proprietary chromatographic techniques.
Product Specs
Introduction
As a member of the GDNF ligand subfamily within the TGF-beta superfamily, Persephin plays a crucial role in the survival and growth of essential dopaminergic and motor neurons. It is also involved in kidney development. However, it's important to note that persephin does not contribute to the survival of peripheral neurons.
Description
Recombinant Human PSPN, produced in E. coli, is a homodimer linked by disulfide bonds. It consists of two chains, each containing 96 amino acids, resulting in a molecular weight of 20.5kDa.
The purification of PSPN is achieved using proprietary chromatographic techniques.
Physical Appearance
White powder, sterile filtered and lyophilized (freeze-dried).
Formulation
Lyophilized from a concentrated solution (0.2µm filtered) in PBS with a pH of 7.4.
Solubility
To reconstitute the lyophilized PSPN, it is recommended to dissolve it in 4mM HCl at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized PSPN remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. Once reconstituted, PSPN should be stored at 4°C for a period of 2 to 7 days. For extended storage, it is advisable to add a carrier protein (0.1% HSA or BSA) and store it below -18°C. Repeated freeze-thaw cycles should be avoided.
Purity
Exceeds 95.0% purity, as determined by: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
Demonstrates full biological activity when compared to the standard. The ED50, determined by a cell proliferation assay using human TT medullary thyroid cancer cells, is less than 10ng/ml. This corresponds to a specific activity greater than 1.0 × 100,000 IU/mg.
Synonyms
Persephin, PSP, PSPN.
Source
Escherichia Coli.
Amino Acid Sequence
ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG.
Product Science Overview
Structure and Expression
Biological Activity
Persephin promotes the survival of mesencephalic, dopaminergic, and motor neurons . Unlike other members of the GDNF family, Persephin does not support the survival of neurons from peripheral ganglia, including sympathetic, parasympathetic, sensory, and enteric neurons . However, it does promote the survival of midbrain dopaminergic neurons after neurotoxic injury and the survival of spinal motor neurons .
Receptor Interaction
Potential Therapeutic Applications
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