PSPH Human

The PSPH gene is located on chromosome 7 in humans and encodes the phosphoserine phosphatase protein . The protein consists of 225 amino acids and has a calculated molecular mass of approximately 25 kDa . It is expressed in various tissues, including the epidermis and hair follicles, and is highly induced in certain skin tumors .

Phosphoserine phosphatase catalyzes the irreversible dephosphorylation of O-phospho-L-serine to L-serine . This reaction is crucial for maintaining adequate levels of L-serine in the body, which can then be used for protein synthesis, nucleotide metabolism, and other vital processes . The enzyme also participates in an exchange reaction between L-serine and L-phosphoserine .

Deficiency in phosphoserine phosphatase activity has been linked to various disorders, including Williams syndrome . Additionally, the enzyme’s expression is altered in certain cancers, such as squamous cell carcinoma (SCC), where it plays a role in cell proliferation and tumor growth .

Recombinant human phosphoserine phosphatase is produced using DNA sequences encoding the human PSPH gene, which are expressed and purified in host systems such as E. coli . The recombinant protein is used in various research applications, including studies on enzyme function, metabolic pathways, and disease mechanisms .

Recombinant human PSPH is typically provided as a lyophilized powder and should be stored under sterile conditions at -20°C to -80°C . It is recommended to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity .