PSPH Antibody

Phosphoserine Phosphatase, Mouse Anti Human
Cat. No.
BT24983
Source
Synonyms
Phosphoserine phosphatase, EC 3.1.3.3, PSP, O-phosphoserine phosphohydrolase, PSPase, L-3-phosphoserine phosphatase, PSPH.
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Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Human Phosphoserine phosphatase (hPSP) is a key enzyme in the phosphorylated pathway of serine biosynthesis, responsible for a significant portion of endogenous L-serine production. hPSP, like other L-3-phosphoserine phosphatases, catalyzes the Mg2+-dependent hydrolysis of L-phosphoserine and facilitates an exchange reaction between L-serine and L-phosphoserine. Recent structural studies have revealed that the enzyme's active site undergoes open-closed conformational changes, driven by a -helical bundle domain. This conformational flexibility is crucial for substrate recognition and hydrolysis through local rearrangements.
Formulation
The antibody is supplied as a 1mg/ml solution in phosphate-buffered saline (PBS) at pH 7.4, with 0.1% sodium azide added as a preservative.
Storage Procedures
For short-term storage (up to 1 month), store the antibody at 4°C. For longer-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This PSPH antibody has been validated for ELISA and Western blot applications to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. For Western blot analysis, a dilution range of 1:1,000 to 1:2,000 is recommended, with a starting dilution of 1:1,000.
Synonyms
Phosphoserine phosphatase, EC 3.1.3.3, PSP, O-phosphoserine phosphohydrolase, PSPase, L-3-phosphoserine phosphatase, PSPH.
Purification Method
PSPH antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P3G12AT.
Immunogen
Anti-human PSPH mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PSPH amino acids 1-225 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Introduction

Phosphoserine Phosphatase (PSPH) is an enzyme encoded by the PSPH gene in humans. This enzyme plays a crucial role in the biosynthesis of L-serine, an amino acid essential for various cellular functions. The mouse anti-human PSPH antibody is a monoclonal antibody derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PSPH .

Gene and Protein Structure

The PSPH gene is located on chromosome 7 in humans and chromosome 5 in mice . The protein encoded by this gene belongs to a subfamily of phosphotransferases and is responsible for the third and final step in L-serine formation. This step involves the magnesium-dependent hydrolysis of L-phosphoserine to L-serine .

Biological Functions

PSPH catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates. L-serine is then utilized in protein synthesis, production of other amino acids, nucleotide metabolism, and glutathione synthesis. Additionally, L-serine can be racemized to D-serine, a neuromodulator .

Clinical Significance

Deficiency in PSPH is associated with several disorders, including Phosphoserine Phosphatase Deficiency and Visual Epilepsy . Homozygous or compound heterozygous mutations in PSPH can cause Neu–Laxova syndrome . Moreover, downregulation of PSPH has been shown to potentiate tumor immune responses, making it a potential target for immune-based cancer therapies .

Applications of Mouse Anti-Human PSPH Antibody

The mouse anti-human PSPH antibody is used in various research applications, including:

  • Western Blotting: To detect PSPH protein levels in different samples.
  • Immunohistochemistry: To study the localization of PSPH in tissues.
  • Flow Cytometry: To analyze PSPH expression in cell populations.

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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