Prolactin Rabbit
Prolactin Rabbit Recombinant
Cat. No.
BT495
Source
Escherichia Coli.
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Prolactin Rabbit Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23007 Dalton.
The Porlactin is purified by proprietary chromatographic techniques.
The Porlactin is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Prolactin, primarily produced by the pituitary gland, is a neuroendocrine hormone with significant roles in lactation, breast cancer development, reproductive function regulation, and immunoregulation. Its synthesis also occurs in other cell types, such as the placenta, brain, and uterus.
Description
Recombinant Rabbit Prolactin, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 200 amino acids with a molecular weight of 23007 Dalton. The purification process involves proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile filtered.
Formulation
The lyophilization of the Prolactin protein was performed from a solution containing 1mg/ml protein and 0.0045mM NaHCO3.
Solubility
For reconstitution of the lyophilized Prolactin, sterile 18 megaohm-centimeter H2O is recommended, at a concentration not less than 100 micrograms/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
While Lyophilized Prolactin demonstrates stability at room temperature for up to 3 weeks, storage in a desiccated state below -18 degrees Celsius is recommended. Following reconstitution, it is advisable to store Prolactin (Prl) at 4 degrees Celsius for a period of 2-7 days. For long-term storage, maintaining a temperature below -18 degrees Celsius is recommended. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is important to avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 98.0% as determined by the following methods:
(a) Size-Exclusion High-Performance Liquid Chromatography (SEC-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
(a) Size-Exclusion High-Performance Liquid Chromatography (SEC-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED50, determined through the dose-dependent stimulation of rat lymphoma Nb2-11 cell proliferation, was found to be less than 0.065 nanograms/ml.
Protein Content
Protein quantitation was performed using two independent methods:
1. UV spectroscopy at 280 nm, utilizing an absorbance value of 0.87 as the extinction coefficient for a 0.1% (equivalent to 1 mg/ml) solution. This value is derived from calculations performed by the PC GENE computer analysis program for protein sequences (developed by IntelliGenetics).
2. Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis, employing a calibrated solution of Prolactin as a reference standard.
1. UV spectroscopy at 280 nm, utilizing an absorbance value of 0.87 as the extinction coefficient for a 0.1% (equivalent to 1 mg/ml) solution. This value is derived from calculations performed by the PC GENE computer analysis program for protein sequences (developed by IntelliGenetics).
2. Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis, employing a calibrated solution of Prolactin as a reference standard.
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Ile-Cys.
Product Science Overview
Prolactin Receptor
The prolactin receptor (PRLR) is a single-pass transmembrane receptor that mediates the actions of prolactin . It is a type 1 cytokine receptor and is best known for promoting lactation in mammals. However, it is also implicated in other functions such as osmoregulation, metabolism, and immune system function .
Recombinant Prolactin in Research
Recombinant prolactin is widely used in research to study its effects on various biological processes. It is particularly valuable in cancer research, as prolactin signaling via PRLR can activate multiple signal transduction pathways in breast cancer cells, including the Jak/Stat, PI3K/Akt, and MAPK pathways . These pathways lead to both pro-proliferative and anti-apoptotic downstream effects, making prolactin a significant factor in tumor growth and progression .
Applications of Recombinant Prolactin
- Cancer Research: Recombinant prolactin is used to investigate its role in promoting the growth of breast cancer and other tumor types. Studies suggest that PRLR activation may promote tumor growth, and defining the precise role of PRLR in cancer remains an area of active investigation .
- Endocrinology: It is used to study the regulation of lactation and other endocrine functions.
- Immunology: Researchers use recombinant prolactin to explore its effects on the immune system.
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