Prolactin Ovine
Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Description
Product Specs
The protein solution, at a concentration of 1mg/ml, was lyophilized with a sodium bicarbonate (NaHCO3) concentration ranging from 0.02% to 0.03%.
To reconstitute the lyophilized Prolactin, it is advised to dissolve it in sterile 18MΩ-cm H2O or 0.4% NaHCO3 solution, adjusted to a pH between 8 and 9. The initial reconstitution should result in a concentration of at least 100µg/ml, which can then be diluted further in other aqueous solutions as needed.
The purity is determined to be greater than 99.0% using the following methods:
(a) Gel filtration analysis.
(b) SDS-PAGE analysis.
Protein quantification is performed using UV spectroscopy at a wavelength of 280 nm. An absorbance value of 0.93 is utilized as the extinction coefficient for a 0.1% (1mg/ml) solution. This specific value is determined by analyzing protein sequences using the PC DNAman computer program.
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.
Product Science Overview
In ruminants, prolactin-related proteins (PRPs) are non-classical members of the prolactin/growth hormone family. These proteins have been identified in the placentas of bovine, caprine, murine, and rat species . In cattle, placental lactogen (PL) and thirteen types of placental PRPs have been reported . In goats, PL has been detected in the placenta, and two newly discovered PRPs were reported as PRL-related molecules .
Ovine prolactin, specifically, is a lactogenic hormone secreted by the adenohypophysis. Besides its major action on lactation, in some species, prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation, and osmoregulation . Prolactin has also been shown to have cytokine-like activities and important immunoregulatory functions .
Recombinant ovine prolactin is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa . The protein is purified using proprietary chromatographic techniques and is available as a sterile, lyophilized (freeze-dried) powder . The recombinant protein is fully biologically active, as evidenced by its ability to induce the proliferation of Nb2 cells .
Recombinant ovine prolactin is used primarily for laboratory research purposes. It is not intended for use as drugs, agricultural or pesticidal products, food additives, or household chemicals . The lyophilized protein is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for future use, with the addition of a carrier protein to prevent freeze-thaw cycles .
