Prolactin Mouse
Prolactin Mouse Recombinant
Cat. No.
BT21
Source
Escherichia Coli.
Synonyms
Mammotropin, Luterotropic hormone, Lutetropin, PRL.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Prolactin Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.5 kDa.
The Prolactin is purified by proprietary chromatographic techniques.
The Prolactin is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Prolactin, a neuroendocrine hormone primarily produced by the pituitary gland, plays a crucial role in lactation. Its production is also observed in other cell types like placenta, brain, and uterus. Apart from its primary function, Prolactin is involved in diverse physiological processes such as breast cancer development, reproductive function regulation, and immunomodulation.
Description
Recombinant Mouse Prolactin, expressed in E.Coli, is a non-glycosylated polypeptide chain consisting of 198 amino acids. With a molecular weight of 22.5 kDa, this single-chain protein is purified using proprietary chromatographic techniques for optimal purity.
Physical Appearance
The purified Mouse Prolactin is provided as a sterile, white lyophilized (freeze-dried) powder, ensuring ease of handling and storage.
Formulation
To ensure stability and preserve its biological activity, Mouse Prolactin is lyophilized from a concentrated solution (1mg/ml) containing 10mM sodium Phosphate buffer (pH 8) and 50mM Nacl.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized Mouse Prolactin in sterile 18MΩ-cm H2O to achieve a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
While the lyophilized Mouse Prolactin remains stable at room temperature for up to 3 weeks, it is recommended to store it desiccated below -18°C to maintain long-term stability. After reconstitution, the solution should be stored at 4°C for 2-7 days. For extended storage, freezing below -18°C is recommended, and adding a carrier protein like 0.1% HSA or BSA can enhance stability. To preserve protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of this Mouse Prolactin is guaranteed to be greater than 95%, as determined by rigorous analytical techniques including RP-HPLC and SDS-PAGE.
Biological Activity
The biological activity of Mouse Prolactin is assessed by its ability to stimulate the proliferation of rat lymphoma, Nb2-11 cells. The ED50, determined through dose-response analysis, is found to be < 0.065 ng/ml. This corresponds to a specific activity of 15,400,000 Units/mg, confirming its potent bioactivity.
Synonyms
Mammotropin, Luterotropic hormone, Lutetropin, PRL.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ile-Cys.
Product Science Overview
Structure and Function
Recombinant Mouse Prolactin
Applications
Recombinant mouse prolactin is used in various research applications, including:
- Cell Proliferation Assays: It is measured in cell proliferation assays using Nb2-11 rat lymphoma cells, with an effective dose (ED50) ranging from 0.1 to 1.0 ng/mL .
- Immunological Studies: Helps in understanding the immune-regulatory roles of prolactin.
- Reproductive Health Research: Used to study its effects on reproductive functions and behaviors.
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