PRDX1 Human

Peroxiredoxin-1 Human Recombinant
Cat. No.
BT24803
Source
Escherichia Coli.
Synonyms
Peroxiredoxin-1, EC 1.11.1.15, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Proliferation-associated gene protein, Natural killer cell-enhancing factor A, NKEF-A, PRDX1, TDPX2, PRDX-1, PAG, PAGA, PRX1, PAGB, PRXI, MSP23, NKEFA.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Peroxiredoxin Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain (1-199) containing 219 amino acids and having a molecular mass of 24 kDa.
The Peroxiredoxin is purified by proprietary chromatographic techniques.

Product Specs

Introduction
PRDX1, a member of the peroxiredoxin family of antioxidant enzymes, plays a crucial role in reducing hydrogen peroxide and alkyl hydroperoxides. This enzyme is vital for protecting red blood cells from reactive oxygen species and preventing tumor formation. PRDX1 exhibits antioxidant properties within cells, contributing to the antiviral activity of CD8(+) T-cells. Additionally, it demonstrates a proliferative effect and is implicated in cancer development and progression. Functioning as a key player in cellular redox regulation, PRDX1 reduces peroxides using reducing equivalents supplied by the thioredoxin system, not glutaredoxin. This enzyme is essential for eliminating peroxides generated during metabolism and participates in the signaling pathways of growth factors and TNF-alpha by controlling intracellular h(2)o(2) concentrations.
Description
Recombinant Human Peroxiredoxin, fused with a 20 amino acid His tag at the N-terminus, is produced in E. coli. This single, non-glycosylated polypeptide chain (1-199) consists of 219 amino acids and has a molecular weight of 24 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The Peroxiredoxin solution is provided at a concentration of 1mg/ml in a buffer containing 20mM Tris-HCl (pH 7.5) and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the Peroxiredoxin is determined to be greater than 90% using SDS-PAGE analysis.
Biological Activity
Enzymatic activity is determined by measuring the amount of hydroperoxide reduced by 1ug of enzyme per minute at 25°C. The specific activity of this Peroxiredoxin is greater than 2,000 pmol/min/ug.
Synonyms
Peroxiredoxin-1, EC 1.11.1.15, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Proliferation-associated gene protein, Natural killer cell-enhancing factor A, NKEF-A, PRDX1, TDPX2, PRDX-1, PAG, PAGA, PRX1, PAGB, PRXI, MSP23, NKEFA.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK.

Product Science Overview

Structure and Function

PRDX1 is a thiol peroxidase that catalyzes the reduction of peroxides, including hydrogen peroxide. It functions similarly to other antioxidant enzymes like catalase and glutathione peroxidase . PRDX1 is part of the 2-Cys peroxiredoxin subfamily, which requires the oxidation of two cysteine residues to activate its enzymatic activity . The enzyme is abundant in the cytosol of mammalian cells and plays a significant role in maintaining intracellular reactive oxygen species (ROS) homeostasis .

Biological Roles
  1. Antioxidant Protection: PRDX1 helps in detoxifying peroxides, thus protecting cells from oxidative stress .
  2. Antiviral Activity: It may contribute to the antiviral activity of CD8 (+) T-cells .
  3. Tumor Suppression: PRDX1 has been shown to play roles in tumor suppression and apoptosis .
  4. Molecular Chaperoning: It also functions as a molecular chaperone, assisting in the proper folding of proteins .
Clinical Significance

PRDX1 is essential for the viability and maintenance of ROS levels in cells. Studies have shown that depletion of PRDX1 results in increased intracellular ROS levels and cell death . PRDX1 knockout mice have been observed to develop severe hemolytic anemia and several malignant cancers, indicating its role in preventing tumor development .

Recombinant PRDX1

Recombinant human PRDX1 is produced using an Escherichia coli expression system. It is used in various research applications, including functional studies and SDS-PAGE . The recombinant protein is stored at -20°C and is typically provided in a buffer containing 20 mM Hepes, pH 7.0, with 10% glycerol and 1 mM DTT .

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