PRDX1 Antibody
Peroxiredoxin-1, Mouse Anti Human
Cat. No.
BT24256
Source
Synonyms
Peroxiredoxin-1, EC 1.11.1.15, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Proliferation-associated gene protein, Natural killer cell-enhancing factor A, NKEF-A, PRDX1, TDPX2, PRDX-1, PAG, PAGA, PRX1, PAGB, PRXI, MSP23, NKEFA.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
PRDX1, a member of the peroxiredoxin family, functions as an antioxidant enzyme by reducing hydrogen peroxide and alkyl hydroperoxides. This enzyme plays a crucial role in protecting red blood cells from reactive oxygen species and preventing tumor formation. PRDX1 exhibits antioxidant properties in cells, contributing to the antiviral activity of CD8(+) T-cells. Additionally, it demonstrates a proliferative effect and is implicated in cancer development and progression. PRDX1 participates in redox regulation within cells, utilizing reducing equivalents from the thioredoxin system, but not glutaredoxin, to decrease peroxide levels. It effectively eliminates peroxides generated during metabolism and participates in signaling pathways of growth factors and TNF-alpha by regulating intracellular hydrogen peroxide concentrations.
Formulation
1 mg/ml in PBS, pH 7.4, with 0.1% Sodium Azide.
Storage Procedures
Store at 4°C for periods up to 1 month. For longer storage, keep at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
PRDX1 antibody has undergone testing by ELISA and Western blot analysis, confirming its specificity and reactivity. However, due to varying applications, it is recommended to titrate the reagent in each investigation to achieve optimal results. For Western blot analysis, a dilution range of 1:1000 to 1:2000 is recommended, with a starting dilution of 1:1000.
Synonyms
Peroxiredoxin-1, EC 1.11.1.15, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Proliferation-associated gene protein, Natural killer cell-enhancing factor A, NKEF-A, PRDX1, TDPX2, PRDX-1, PAG, PAGA, PRX1, PAGB, PRXI, MSP23, NKEFA.
Purification Method
PRDX1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1D8AT.
Immunogen
Anti-human PRDX1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PRDX1 amino acids 1-199 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Introduction
Peroxiredoxin-1 (PRDX1) is a member of the peroxiredoxin family of antioxidant enzymes. These enzymes play a crucial role in reducing hydrogen peroxide and alkyl hydroperoxides, thereby protecting cells from oxidative damage . The mouse anti-human PRDX1 antibody is a tool used in various research applications to detect and study the PRDX1 protein in human samples.
Structure and Function of PRDX1
PRDX1 is a thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively . This activity is vital for cell protection against oxidative stress. PRDX1 also acts as a sensor for hydrogen peroxide-mediated signaling events, participating in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating intracellular concentrations of hydrogen peroxide .
Biological Significance
PRDX1 has several important biological roles:
- Antioxidant Protection: PRDX1 helps in detoxifying peroxides, thus protecting cells from oxidative damage .
- Antiviral Activity: It may contribute to the antiviral activity of CD8(+) T-cells .
- Cancer Development: PRDX1 may have a proliferative effect and play a role in cancer development or progression .
- Cardiovascular Protection: In mice, the deletion of PRDX1 leads to excessive endothelial activation and inflammation, resulting in early atherosclerosis, indicating its protective role in vascular inflammation and atherogenesis .
Mouse Anti-Human PRDX1 Antibody
Applications in Research
- Western Blotting: The antibody is used to detect PRDX1 in cell lysates from human, mouse, and rat samples .
- ELISA: It is employed in direct ELISAs to measure PRDX1 levels with minimal cross-reactivity with other peroxiredoxins .
- Cancer Research: Given PRDX1’s role in cancer, the antibody is used to study its expression in various cancer cell lines .
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