PRDX1 Mouse

Peroxiredoxin-1 Mouse Recombinant
Cat. No.
BT24879
Source
Sf9, Baculovirus cells.
Synonyms
Peroxiredoxin-1, Macrophage 23 kDa stress protein, Osteoblast-specific factor 3, OSF-3, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PRDX1 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 207 amino acids (1-199a.a.) and having a molecular mass of 23.2kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
PRDX1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
PRDX1, a member of the peroxiredoxin antioxidant enzyme family, plays a vital role in reducing hydrogen peroxide and alkyl hydroperoxides. It is a crucial protector of red blood cells against reactive oxygen species and contributes to tumor prevention. PRDX1 exhibits antioxidant properties within cells and enhances the antiviral activity of CD8(+) T-cells. Furthermore, it demonstrates a proliferative effect and is implicated in cancer development and progression. As a key player in cellular redox regulation, PRDX1 reduces peroxides using reducing equivalents provided by the thioredoxin system, not glutaredoxin. It effectively eliminates peroxides generated during metabolism and participates in the signaling cascades of growth factors and TNF-alpha by regulating intracellular h(2)o(2) concentrations.
Description
Recombinant Mouse PRDX1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular weight of 23.2kDa (appears at approximately 18-28kDa on SDS-PAGE). It comprises 207 amino acids (1-199a.a.). This PRDX1 protein is expressed with an 8 amino acid His tag at the C-terminus and purified through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The PRDX1 protein solution is provided at a concentration of 0.5mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the PRDX1 protein is greater than 90.0% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of PRDX1 is measured to be greater than 2,500 pmol/min/ug. This enzymatic activity refers to the amount of hydroperoxide reduced by 1ug of enzyme at 25°C per minute.
Synonyms
Peroxiredoxin-1, Macrophage 23 kDa stress protein, Osteoblast-specific factor 3, OSF-3, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
MSSGNAKIGY PAPNFKATAV MPDGQFKDIS LSEYKGKYVV FFFYPLDFTF VCPTEIIAFS DRADEFKKLN CQVIGASVDS HFCHLAWINT PKKQGGLGPM NIPLISDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITINDLPVG RSVDEIIRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVNK SKEYFSKQKL EHHHHHH.

Product Science Overview

Structure and Expression

The recombinant mouse Peroxiredoxin-1 protein is typically expressed with a C-terminal polyhistidine tag, which facilitates its purification. The protein consists of 199 amino acids and has a molecular weight of approximately 23.2 kDa . It is expressed in various host systems, including baculovirus and E. coli .

Function and Mechanism

Peroxiredoxin-1 functions as a thioredoxin peroxidase, reducing peroxides through a conserved cysteine residue in its active site. This reduction process is essential for maintaining cellular redox balance and protecting cells from oxidative stress . The enzyme’s activity is defined by its ability to reduce hydroperoxides, with a specific activity greater than 2,500 pmol/min/µg .

Biological Significance

Peroxiredoxin-1 is constitutively expressed in most human cells and is upregulated upon serum stimulation in both untransformed and transformed cells . It plays a significant role in the antiviral activity of CD8+ T-cells and may contribute to cancer development and progression due to its proliferative effects . Overexpression of Peroxiredoxin-1 has been associated with poor prognosis in several types of human cancers .

Applications

Recombinant mouse Peroxiredoxin-1 is widely used in research to study its antioxidant properties, role in redox regulation, and involvement in various diseases. It is also utilized in enzyme activity assays and structural studies .

Storage and Handling

The recombinant protein is typically stored at -20°C to -80°C to maintain its stability. It is recommended to avoid repeated freeze-thaw cycles to preserve its functionality .

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PRDX1 Human
Peroxiredoxin-1 Human Recombinant
Cat. No.
BT24803
Source
Escherichia Coli.
THE BIOTEK
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