PPM1G Human, 546 a.a.

Protein Phosphatase 1G 546 a.a. Human Recombinant
Cat. No.
BT173
Source
E.coli.
Synonyms
Protein Phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2C GAMMA, EC 3.1.3.16, Protein phosphatase 2C isoform gamma, PP2C-gamma, Protein phosphatase magnesium-dependent 1 gamma, Protein phosphatase 1C, PPM1G, PPM1C.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PPM1G Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 566 amino acids (1-546) and having a molecular mass of 61.4 kDa.
The PPM1G is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
PPM1G, a member of the PP2C family of Ser/Thr protein phosphatases, acts as a negative regulator of cell stress response pathways. It is involved in the dephosphorylation of Pre-mRNA splicing factors, essential for the formation of functional spliceosomes. PPM1G plays a role in regulating cell cycle progression. Additionally, it mediates histone dephosphorylation/exchange in response to DNA damage or during checkpoint recovery in higher eukaryotes. PPM1G induces the degradation of p21/WAF1 through a proteasome-dependent mechanism. Furthermore, Protein phosphatase 1G regulates the assembly and function of the beta-catenin degradation complex.
Description
Recombinant PPM1G Human, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 566 amino acids (1-546). It has a molecular weight of 61.4 kDa. The PPM1G protein is fused to a 20 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
PPM1G is supplied as a 1 mg/ml solution in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl, 0.1mM PMSF, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 85% by SDS-PAGE analysis.
Synonyms
Protein Phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2C GAMMA, EC 3.1.3.16, Protein phosphatase 2C isoform gamma, PP2C-gamma, Protein phosphatase magnesium-dependent 1 gamma, Protein phosphatase 1C, PPM1G, PPM1C.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD

Product Science Overview

Expression and Tissue Distribution

PPM1G is widely expressed in human tissues, with the highest levels found in the testis, skeletal muscle, and heart . The enzyme is localized in the cytoplasm and plays a crucial role in the dephosphorylation of pre-mRNA splicing factors, which is essential for the formation of functional spliceosomes .

Biological Functions

PPM1G is involved in several key biological processes:

  1. Cell Cycle Regulation: PPM1G plays a role in regulating cell cycle progression by dephosphorylating specific substrates involved in cell cycle control .
  2. Stress Response: Members of the PP2C family, including PPM1G, are known to be negative regulators of cell stress response pathways .
  3. Pre-mRNA Splicing: PPM1G is responsible for the dephosphorylation of pre-mRNA splicing factors, which is crucial for the assembly and function of the spliceosome .
Recombinant Human PPM1G

The recombinant human PPM1G protein, specifically the C-terminal fragment (Met 317-Asp 546), is expressed in Escherichia coli (E. coli) and is tagged with a polyhistidine tag at the N-terminus . This recombinant protein comprises 241 amino acids and has a predicted molecular mass of approximately 26.6 kDa . It is supplied in a sterile buffer containing 50mM Tris, 1mM DTT, and 20% glycerol at pH 7.5 .

Stability and Storage

The recombinant human PPM1G protein is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions . It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles, which can affect its stability and biological activity .

Research Applications

PPM1G is a valuable tool in research areas focusing on phosphatases and their regulatory mechanisms . It has been studied extensively for its role in cell cycle regulation, stress response, and pre-mRNA splicing . The recombinant form of PPM1G is used in various biochemical assays to understand its function and regulation in cellular processes .

References
  1. Sino Biological - Recombinant Human PPM1G Protein
  2. Sino Biological - Recombinant Human PPM1G Protein

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