PPM1G Antibody
Protein Phosphatase 1G, Mouse Anti Human
Cat. No.
BT23907
Source
Synonyms
Protein Phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2C GAMMA, EC 3.1.3.16, Protein phosphatase 2C isoform gamma, PP2C-gamma, Protein phosphatase magnesium-dependent 1 gamma, Protein phosphatase 1C, PPM1G, PPM1C.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
PPM1G, a member of the PP2C family of Ser/Thr protein phosphatases, acts as a negative regulator of cellular stress response pathways. It plays a crucial role in dephosphorylating Pre-mRNA splicing factors, essential components for the assembly of functional spliceosomes. PPM1G is involved in regulating cell cycle progression. In response to DNA damage or during checkpoint recovery in higher eukaryotes, PPM1G mediates histone dephosphorylation and exchange. It induces proteasome-dependent degradation of p21/WAF1. Additionally, Protein phosphatase 1G is involved in regulating the assembly and function of the beta-catenin degradation complex.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer consisting of PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage of up to 1 month, the antibody should be kept at 4°C. For extended storage, it is recommended to store at -20°C. Repeated freeze-thaw cycles should be avoided.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and 1 month when stored at 4°C.
Applications
The PPM1G antibody has undergone rigorous testing using ELISA, Western blot, and immunohistochemistry analysis to ensure its specificity and reactivity. However, it is essential to optimize the antibody concentration for each specific application to achieve optimal results. For Western blot analysis, the recommended dilution range is 1:1,000 to 1:2,000, with a starting dilution of 1:1,000. For immunohistochemistry analysis, the recommended dilution range is 1:50 to 1:100, with a starting dilution of 1:50.
Synonyms
Protein Phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2C GAMMA, EC 3.1.3.16, Protein phosphatase 2C isoform gamma, PP2C-gamma, Protein phosphatase magnesium-dependent 1 gamma, Protein phosphatase 1C, PPM1G, PPM1C.
Purification Method
PPM1G antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
Pk1G6AT.
Immunogen
Anti-human PPM1G mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPM1G amino acids 317-546 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Gene and Protein Information
Function and Mechanism
PPM1G is responsible for the dephosphorylation of pre-mRNA splicing factors, which is crucial for the formation of a functional spliceosome . This process is essential for the regulation of cell cycle progression . The enzyme exhibits protein serine/threonine phosphatase activity and requires Mg2+ or Mn2+ ions as cofactors .
Biological Significance
PPM1G plays a significant role in various cellular processes, including:
- Regulation of Cell Cycle: It is involved in the regulation of cell cycle progression .
- Protein Dephosphorylation: It dephosphorylates specific proteins, which is a critical step in many signaling pathways .
- Cell Stress Response: As a member of the PP2C family, it acts as a negative regulator of cell stress response pathways .
Mouse Anti Human PPM1G Antibody
The Mouse Anti Human PPM1G Antibody is a monoclonal antibody derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPM1G amino acids 317-546 purified from E. coli . This antibody is used in various research applications, including immunohistochemistry, immunoprecipitation, and Western blotting .
Applications in Research
The PPM1G antibody is widely used in research to study the protein’s role in cellular processes and its involvement in diseases. It helps in:
- Identifying Protein Interactions: By using immunoprecipitation techniques, researchers can identify proteins that interact with PPM1G.
- Studying Protein Localization: Immunohistochemistry allows researchers to determine the localization of PPM1G within cells.
- Analyzing Protein Expression: Western blotting is used to analyze the expression levels of PPM1G in different tissues and under various conditions .
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