PPM1F Human
Protein Phosphatase 1F Human Recombinant
Cat. No.
BT54
Source
E.coli.
Synonyms
Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, KIAA0015, POPX2, PPM1F, CAMKP, FEM-2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
PPM1F Human Recombinant produced in E. coli is a single polypeptide
chain containing 477 amino acids (1-454) and having a molecular mass of
52kDa.
PPM1F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Protein Phosphatase 1F (PPM1F), a member of the PP2C family of Ser/Thr protein phosphatases, acts as a negative regulator of cellular stress response pathways. PPM1F interacts with Rho guanine nucleotide exchange factors (PIX), thereby inhibiting the activity of p21-activated kinase 1 (PAK), a kinase involved in mediating downstream effects of Rho GTPases. Notably, PPM1F dephosphorylates Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) and has been identified as a promoter of apoptosis.
Description
Recombinant Human PPM1F, expressed in E. coli, is a single polypeptide chain comprising 477 amino acids (residues 1-454) with a molecular weight of 52 kDa. A 23 amino acid His-tag is fused to the N-terminus of PPM1F, which is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
PPM1F is supplied as a 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, KIAA0015, POPX2, PPM1F, CAMKP, FEM-2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ
KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG
SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ
SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD
PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK
RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG
GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP
HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE
GDPQAEGRRQ DLPSSLPEPE TQAPPRS.
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