PPM1F Antibody
Protein Phosphatase 1F, Mouse Anti Human
Cat. No.
BT23806
Source
Synonyms
Protein phosphatase 1F, Ca(2+)/calmodulin-dependent
protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2,
Protein fem-2 homolog, hFem-2, KIAA0015, POPX2, PPM1F, CAMKP, FEM-2.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Protein Phosphatase 1F (PPM1F), a member of the PP2C family of Ser/Thr protein phosphatases, acts as a negative regulator of cell stress response pathways. PPM1F interacts with Rho guanine nucleotide exchange factors (PIX), thereby inhibiting the activity of p21-activated kinase 1 (PAK), a protein kinase involved in mediating the downstream effects of Rho GTPases. Notably, PPM1F dephosphorylates Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) and has been identified as a promoter of apoptosis.
Physical Appearance
Sterile, colorless solution.
Formulation
The antibody is supplied at a concentration of 1 mg/ml in a solution containing PBS (pH 7.4), 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store at 4°C. For extended storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has been validated for ELISA and Western blot analysis, demonstrating its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. A starting dilution of 1:1000 is recommended.
Synonyms
Protein phosphatase 1F, Ca(2+)/calmodulin-dependent
protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2,
Protein fem-2 homolog, hFem-2, KIAA0015, POPX2, PPM1F, CAMKP, FEM-2.
Purification Method
PPM1F antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT6D11AT.
Immunogen
Anti-human PPM1F mAb,
clone PAT6D11AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human PPM1F protein 1-454
amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Gene and Protein Information
- Gene Location: In humans, the PPM1F gene is located on chromosome 22 (22q11.22), while in mice, it is found on chromosome 16 .
- Protein Function: PPM1F dephosphorylates and deactivates CaM-kinase II, IV, and I, which are activated upon phosphorylation by CaM-kinase kinase. This dephosphorylation process is crucial for regulating various cellular functions .
Biological Role and Mechanism
PPM1F interacts with Rho guanine nucleotide exchange factors (PIX), thereby blocking the effects of p21-activated kinase 1 (PAK), a protein kinase that mediates biological effects downstream of Rho GTPases . One of the substrates of PPM1F is Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II), which plays a role in various cellular processes, including apoptosis .
Clinical Significance
Research and Applications
The mouse anti-human PPM1F antibody is used in various research applications to study the enzyme’s function and its role in different cellular pathways. This antibody helps in identifying and quantifying PPM1F in human tissues, aiding in the understanding of its biological significance and potential therapeutic targets .
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