PPA1 Human
Pyrophosphatase-1 Human Recombinant
Cat. No.
BT30882
Source
E.coli.
Synonyms
Pyrophosphatase (inorganic) 1, PP, PP1, IOPPP, SID6-8061, Pyrophosphate phospho-hydrolase, PPase, cytosolic inorganic pyrophosphatase, diphosphate phosphohydrolase, inorganic diphosphatase, EC 3.6.1.1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PPA1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 313 amino acids (1-289) and having a molecular mass of 35.2kDa.
PPA1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PPA1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
PPA1, a member of the PPase family, catalyzes the irreversible conversion of pyrophosphate into two phosphate ions. This hydrolysis is crucial for driving reactions forward in various biochemical pathways. For instance, during lipid degradation, inorganic pyrophosphatase facilitates the activation of fatty acids for oxidation by rapidly hydrolyzing pyrophosphate.
Description
Recombinant human PPA1, expressed in E.coli, is a non-glycosylated polypeptide chain consisting of 313 amino acids (1-289). With a molecular weight of 35.2kDa, this protein is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile solution.
Formulation
The PPA1 solution is provided at a concentration of 0.5mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 200mM NaCl, 2mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C with the addition of a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of PPA1 is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Pyrophosphatase (inorganic) 1, PP, PP1, IOPPP, SID6-8061, Pyrophosphate phospho-hydrolase, PPase, cytosolic inorganic pyrophosphatase, diphosphate phosphohydrolase, inorganic diphosphatase, EC 3.6.1.1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSGFST EERAAPFSLE YRVFLKNEKG QYISPFHDIP IYADKDVFHM VVEVPRWSNA KMEIATKDPL NPIKQDVKKG KLRYVANLFP YKGYIWNYGA IPQTWEDPGH NDKHTGCCGD NDPIDVCEIG SKVCARGEII GVKVLGILAM IDEGETDWKV IAINVDDPDA ANYNDINDVK RLKPGYLEAT VDWFRRYKVP DGKPENEFAF NAEFKDKDFA IDIIKSTHDH WKALVTKKTN GKGISCMNTT LSESPFKCDP DAARAIVDAL PPPCESACTV PTDVDKWFHH QKN.
Product Science Overview
Structure and Expression
Human recombinant PPA1 is typically expressed in Escherichia coli (E. coli) systems. The recombinant form of this enzyme is often tagged with a His-tag at the N-terminus to facilitate purification. The full-length protein consists of 289 amino acids and has a molecular mass of approximately 33 kDa .
Function and Importance
PPA1 is essential for numerous cellular processes, including:
- DNA Synthesis: The hydrolysis of PPi to Pi is a critical step in DNA polymerization, ensuring the continuation of the DNA synthesis process .
- Bone Formation: PPA1 activity is involved in bone mineralization by regulating the levels of pyrophosphate, which is an inhibitor of hydroxyapatite crystal formation .
- Energy Metabolism: The enzyme’s activity is linked to ATP hydrolysis, where PPi is a byproduct. By converting PPi to Pi, PPA1 helps maintain the balance of phosphate ions within the cell .
Biochemical Properties
Recombinant human PPA1 is characterized by its high specific activity, with the ability to hydrolyze more than 50,000 pmol of pyrophosphate per minute per microgram of protein under optimal conditions . The enzyme is typically supplied in a solution containing Tris, NaCl, glycerol, and DTT to ensure stability and activity .
Applications
Recombinant PPA1 is widely used in research and industrial applications, including:
- Biochemical Assays: To study enzyme kinetics and the role of pyrophosphate in various metabolic pathways.
- Structural Biology: For crystallization and structural analysis to understand the enzyme’s mechanism of action.
- Biotechnology: In the production of nucleotides and other phosphate-containing compounds where the removal of pyrophosphate is necessary .
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