Pyrophosphatase-1, also known as Inorganic Pyrophosphatase (PPA1), is an enzyme that plays a crucial role in cellular metabolism by catalyzing the hydrolysis of inorganic pyrophosphate (PPi) into two orthophosphate (Pi) molecules. This reaction is essential for various biochemical processes, including DNA synthesis, protein synthesis, and energy metabolism. The enzyme is ubiquitously expressed in various organisms, including humans, mice, and rats.
PPA1 is a member of the pyrophosphatase family of enzymes and typically has a molecular weight ranging from 32 to 36 kDa . The enzyme requires magnesium ions (Mg²⁺) as cofactors for its catalytic activity. The hydrolysis of PPi by PPA1 is a highly exergonic reaction, which helps drive many biosynthetic reactions forward by removing PPi, a byproduct that can inhibit these processes if accumulated.
The activity of PPA1 is vital for maintaining cellular homeostasis. By hydrolyzing PPi, PPA1 prevents the accumulation of this molecule, which can be detrimental to cellular functions. The enzyme’s activity is particularly important in tissues with high metabolic rates, such as the liver, brain, and muscle.
The Mouse Anti Human Pyrophosphatase-1 antibody is a monoclonal antibody designed to specifically detect human PPA1. This antibody is commonly used in various research applications, including Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA). The antibody is produced by immunizing mice with recombinant human PPA1 protein, followed by hybridoma technology to generate monoclonal antibody-producing cells .
The Mouse Anti Human Pyrophosphatase-1 antibody is a valuable tool in biomedical research. It allows scientists to study the expression and regulation of PPA1 in different tissues and under various physiological and pathological conditions. For example, researchers can use this antibody to investigate the role of PPA1 in cancer, neurodegenerative diseases, and metabolic disorders.