PLGF2 Human
Escherichia Coli.
PIGF, PGF, PlGF-2, PLGF-2.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Placental Growth Factor-2 Human Recombinant produced in E.Coli is a non-glycosylated, disulfide-linked homodimer consisting of 2x152 amino acid polypeptide chains, having a total molecular mass of approximately 34.6kDa.
PLGF2 is purified by proprietary chromatographic techniques.
Product Specs
PLGF is a growth factor that plays a role in angiogenesis and endothelial cell growth, by encouraging their proliferation and movement. It interacts with the receptor known as vegfr-1/flt1. PLGF-2 specifically binds to neuropilin-1 and 2 in a manner that depends on the presence of other molecules.
Recombinant Human Placental Growth Factor-2, produced in E. coli, is a homodimer protein that lacks glycosylation. Each polypeptide chain consists of 152 amino acids, linked by disulfide bonds, resulting in a total molecular weight of approximately 34.6kDa.
The purification of PLGF2 is achieved through specific chromatographic techniques developed by the company.
The product appears as a sterile, white powder that has been lyophilized (freeze-dried) and filtered for sterility.
The product is lyophilized from a concentrated solution in PBS with a pH of 7.4 and 0.02% Tween-20, which has been filtered through a 0.2µm filter.
To reconstitute the lyophilized Placental Growth Factor-2, it is recommended to dissolve it in sterile 18MΩ-cm H₂O to a concentration of at least 100µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Lyophilized PLGF2, while stable at room temperature for up to 3 weeks, should ideally be stored in a dry environment below -18°C. After reconstitution, Placental Growth Factor-2 should be stored at 4°C for a period of 2-7 days. For long-term storage, it is recommended to keep it below -18°C.
Avoid repeated cycles of freezing and thawing.
The purity of the product is greater than 97.0%, as determined by the following methods:
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
The biological activity of the product is assessed by its ability to induce chemotaxis in human monocytes, within a concentration range of 5.0 to 50 ng/ml.
PIGF, PGF, PlGF-2, PLGF-2.
Escherichia Coli.
LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR.
Product Science Overview
Placental Growth Factor-2 (PlGF-2) is a member of the vascular endothelial growth factor (VEGF) family, which plays a crucial role in angiogenesis and endothelial cell growth. PlGF-2 is particularly significant in the context of placental development and function. This article delves into the structure, function, and applications of human recombinant PlGF-2.
PlGF-2 is one of the isoforms of Placental Growth Factor (PlGF), which is produced through alternative splicing of the PlGF gene. The human PlGF gene can produce at least three isoforms: PlGF-1, PlGF-2, and PlGF-3, containing 131, 152, and 203 amino acids, respectively . PlGF-2 is characterized by the presence of a heparin-binding domain, which distinguishes it from PlGF-1 .
PlGF-2 primarily functions in the regulation of angiogenesis, the process by which new blood vessels form from pre-existing vessels. This is particularly important during pregnancy, where PlGF-2 supports the growth and differentiation of trophoblasts, the cells that form the outer layer of the blastocyst and provide nutrients to the embryo . PlGF-2 binds to the VEGF receptor-1 (VEGFR-1), also known as Flt-1, promoting endothelial cell proliferation and migration .
Human recombinant PlGF-2 is produced using recombinant DNA technology, which involves inserting the PlGF-2 gene into a suitable expression system, such as E. coli or mammalian cells. The recombinant protein is then purified to achieve high levels of purity and biological activity . This process ensures that the recombinant PlGF-2 closely mimics the natural protein in terms of structure and function.
Recombinant PlGF-2 has several applications in research and medicine:
- Angiogenesis Research: PlGF-2 is used to study the mechanisms of blood vessel formation and the role of growth factors in this process.
- Therapeutic Development: PlGF-2 is being investigated for its potential therapeutic applications in conditions characterized by impaired angiogenesis, such as ischemic heart disease and peripheral artery disease.
- Pregnancy Complications: PlGF-2 levels are studied in the context of pregnancy-related disorders, such as preeclampsia, to understand their role in the pathophysiology of these conditions .
