PLGF2 Human, HEK
HEK293 cells.
PIGF, PGF, PlGF-2, PLGF-2.
Sterile Filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
PLGF2 Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 19-170) containing 158 amino acids and having a molecular mass of 18.1kDa. PLGF2 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
PIGF, PGF, PlGF-2, PLGF-2.
HEK293 cells.
LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RRHHHHHH.
Product Science Overview
Placental Growth Factor-2 (PLGF-2) is a member of the vascular endothelial growth factor (VEGF) subfamily, which plays a crucial role in angiogenesis and vasculogenesis. PLGF-2 is a homodimeric glycoprotein primarily expressed in the placental trophoblast during pregnancy, but it is also present in various other tissues such as the villous trophoblast .
PLGF-2 is encoded by the PGF gene, which is located on human chromosome 14q24-q31 . The PGF gene produces multiple isoforms of the protein, including PLGF-1 and PLGF-2. These isoforms differ in their structure and function. PLGF-2, in particular, is known for its role in enhancing the production of peripheral blood mononuclear cell-vascular endothelial growth factor (PBMC-VEGF), which is important for various physiological processes .
PLGF-2 is a homodimeric glycoprotein with a molecular weight of approximately 46-50 kDa . When expressed in HEK 293 cells, the recombinant form of PLGF-2 is glycosylated and has a calculated molecular weight of 20.2 kDa, but it migrates as a 28-33 kDa protein due to glycosylation . The protein is typically tagged with a 6-His tag at the C-terminus for purification purposes .
PLGF-2 acts as a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and is implicated in various physiological functions such as tumor angiogenesis, wound healing, and the recruitment of hematopoietic progenitors . It is also associated with the pathogenesis of rheumatoid arthritis and other arthropathies . Additionally, PLGF-2 interacts with soluble fms-like tyrosine kinase-1 (sFlt-1), an anti-angiogenic factor, which inhibits its activity. This interaction is particularly relevant in the context of pre-eclampsia, where PLGF levels are lowered and sFlt-1 levels are increased in placental tissue .
The recombinant form of PLGF-2 is produced in HEK 293 cells, a human embryonic kidney cell line commonly used for the expression of recombinant proteins. The protein is expressed as a lyophilized powder and is typically purified using proprietary chromatographic techniques . The recombinant PLGF-2 is used in various research applications, including studies on angiogenesis, cancer, and inflammatory diseases.
