PF 4 Mouse
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Product Specs
Platelet factor 4 (PF4), also known as CXCL4, is a protein composed of 70 amino acids. It is stored in and released from the alpha granules of activated platelets. Primarily, PF4 appears to regulate coagulation by neutralizing molecules found on the lining of blood vessels, known as the endothelium. This action helps prevent excessive clotting. Additionally, PF4 acts as a chemoattractant, attracting neutrophils and fibroblasts to sites of injury or inflammation. This suggests a role for PF4 in wound healing and inflammatory responses. Oncostatin-A belongs to the same chemokine family as PF4, the CXC chemokine family.
The Mouse CXCL4 protein was freeze-dried from a 0.2µm filtered solution at a concentration of 20mM PB, pH 7.4, and 1.5M NaCl.
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
The biological activity of CXCL4 is assessed through its ability to attract human neutrophils. This chemoattractant activity is measured within a concentration range of 10-100ng/ml.
Product Science Overview
PF-4 is an 8 kDa protein consisting of 70 amino acids . The recombinant mouse CXCL4/PF4 protein is typically expressed in E. coli and purified to a high degree of purity, often greater than 97% as determined by SDS-PAGE under reducing conditions . The protein is lyophilized from a 0.2 μm filtered solution in PBS and can be reconstituted in sterile PBS for use in various applications .
PF-4/CXCL4 has several biological functions depending on the cell type:
- Procoagulant and Anticoagulant Activities: PF-4 can bind heparin with high affinity, neutralizing its anticoagulant effect. It also inhibits factor XII and vitamin K-dependent coagulation factors, while stimulating activated protein C generation .
- Antiangiogenic Activity: PF-4 is a potent inhibitor of angiogenesis. It can inhibit endothelial cell migration, proliferation, and in vivo angiogenesis by interfering with the angiogenic effects of growth factors such as FGF and VEGF .
- Immunomodulatory Activities: PF-4 plays a role in hematopoiesis and immune cell modulation. It can activate granulocytes and induce monocytes to differentiate into macrophages and antigen-presenting cells .
PF-4 is deposited early in atherosclerotic lesions and is associated with plaque progression. Genetic deletion of CXCL4 in Apo-E-/- mice is accompanied by reduced atherogenesis . Additionally, PF-4 has been identified as a potent tumor inhibitor due to its ability to inhibit endothelial cell functions .
Recombinant mouse CXCL4/PF4 is used in various research applications, including:
- Inhibition of FGF-dependent proliferation: It is measured by its ability to inhibit the FGF basic-dependent proliferation of HUVEC (human umbilical vein endothelial cells), with an ED50 for this effect ranging from 2-10 μg/mL .
- Studies on angiogenesis and coagulation: Due to its antiangiogenic and procoagulant properties, PF-4 is widely used in studies related to these fields .
