PF4 Human, His
Platelet Factor-4 Human Recombinant (CXCL4), His Tag
Cat. No.
BT22610
Source
Escherichia Coli.
Synonyms
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
Appearance
Sterile Filtered colorless clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PF4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 91 amino acids (32-101 a.a) and having a molecular mass of 10kDa.
PF4 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PF4 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Platelet factor-4 (PF4) is a protein released from activated platelets that plays a role in blood clotting, inflammation, and wound healing. It binds to heparin and neutralizes its anti-clotting effects. PF4 also attracts immune cells like neutrophils and fibroblasts to injury sites. Recombinant human PF4 is used in research to study heparin-induced thrombocytopenia, angiogenesis inhibition, and other related processes.
Description
Recombinant human PF4 is a non-glycosylated protein with a molecular weight of 10 kDa. It consists of a single polypeptide chain of 91 amino acids (residues 32-101) and includes a 21 amino acid His-tag at the N-terminus for purification. The protein is produced in E. coli and purified using chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The PF4 protein solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 50% glycerol, and 2mM DTT.
Stability
For short-term storage (up to 4 weeks), store the PF4 protein at 4°C. For long-term storage, freeze the protein at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the PF4 protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEAEEDGDLQ CLCVKTTSQV RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIIKKLLE S.
Product Science Overview
Structure and Composition
Physiological Role
The primary physiological role of PF-4 is to neutralize heparin-like molecules on the endothelial surface of blood vessels . This action inhibits local antithrombin III activity, thereby promoting coagulation . PF-4 is also a strong chemoattractant for neutrophils and fibroblasts, suggesting a role in inflammation and wound repair .
Biological Activity
Recombinant human PF-4 exhibits several biological activities:
Applications in Research
Recombinant PF-4 is used in various research applications, including:
- Chemoattraction Assays: To study the migration of fibroblasts and neutrophils .
- Coagulation Studies: To investigate the role of PF-4 in blood coagulation and its interaction with heparin .
- Inflammation and Wound Healing: To explore the involvement of PF-4 in inflammatory responses and tissue repair .
Storage and Handling
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