Placental Lactogen Ovine

Placental Lactogen Ovine Recombinant
Cat. No.
BT29314
Source
Escherichia Coli.
Synonyms
Chorionic Somatomammotropin Hormone 1, CSH1, CS-1, hCS, PL.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Placental Lactogen Ovine Recombinant, is a single polypeptide chain containing 199 amino acids and an additional Ala at the N-terminus having a molecular mass of 23 kDa.
Placental Lactogen Recombinant is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Placental Lactogen, also known as chorionic somatomammotropin, is a polypeptide hormone produced by the syncytiotrophoblasts of the placenta. This hormone exhibits both growth hormone and prolactin-like activities, influencing growth, lactation, and the production of luteal steroids. In women, placental lactogen secretion commences shortly after implantation, escalating to a gram or more daily during late pregnancy. Additionally, placental lactogen acts as an insulin antagonist. Notably, Ovine Placental Lactogen can activate human and other heterologous growth hormone receptors, but not ruminant growth hormone receptors.
Description
Recombinant Ovine Placental Lactogen is a single-chain polypeptide composed of 199 amino acids, with an additional alanine residue at the N-terminus, resulting in a molecular weight of 23 kDa. The purification of Recombinant Placental Lactogen is achieved through proprietary chromatographic methods.
Physical Appearance
White, sterile-filtered lyophilized powder.
Formulation
The lyophilization of the protein was carried out from a concentrated solution (1 mg/ml) containing 0.02-0.03% sodium bicarbonate.
Solubility
For reconstitution of the lyophilized Placental Lactogen, sterile 18 MΩ-cm H2O is recommended, with a minimum concentration of 100 µg/ml. Further dilutions can be made using other aqueous solutions.
Stability
Lyophilized Ovine Placental Lactogen, while stable at room temperature for up to 3 weeks, should ideally be stored desiccated at temperatures below -18°C. After reconstitution, Placental Lactogen should be kept refrigerated at 4°C for 2-7 days. For long-term storage, freezing below -18°C is recommended. It is advised to add a carrier protein (0.1% HSA or BSA) for long-term storage and to avoid repeated freeze-thaw cycles.
Purity
The purity is greater than 98.0%, as determined by: (a) SEC-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
Ovine Placental Lactogen demonstrates biological activity through its ability to stimulate the proliferation of Nb2 cells.
Protein Content
Protein content is determined using UV spectroscopy at a wavelength of 280 nm. An absorbance value of 0.85 is employed as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is derived from the PC GENE computer analysis program for protein sequences (IntelliGenetics).
Synonyms
Chorionic Somatomammotropin Hormone 1, CSH1, CS-1, hCS, PL.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Gln-His-Pro-Pro.

Product Science Overview

Introduction

Placental lactogen (PL) is a hormone produced by the placenta during pregnancy. It plays a crucial role in regulating the metabolic state of the mother to support fetal growth and development. Ovine placental lactogen (oPL) is the specific form of this hormone found in sheep. Recombinant ovine placental lactogen (roPL) refers to the artificially synthesized version of this hormone, produced using recombinant DNA technology.

Structure and Function

Ovine placental lactogen is a member of the prolactin/growth hormone family. It shares structural similarities with both prolactin (PRL) and growth hormone (GH), allowing it to bind to their respective receptors and exert similar biological effects . The primary function of oPL is to modulate maternal metabolism to ensure an adequate supply of nutrients to the developing fetus. It also plays a role in mammary gland development and lactation.

Recombinant Production

The production of recombinant ovine placental lactogen involves the insertion of the oPL gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of the hormone in a controlled environment. The recombinant hormone is then purified and characterized to ensure it retains its biological activity .

Applications

Recombinant ovine placental lactogen has several applications in research and agriculture. In research, it is used to study the physiological roles of placental lactogen and its interactions with other hormones. In agriculture, roPL can be used to enhance reproductive performance and milk production in livestock.

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