Placental Lactogen Caprine
Placental Lactogen Caprine Recombinant
Cat. No.
BT29213
Source
Escherichia Coli.
Synonyms
Chorionic Somatomammotropin Hormone 1, CSH1, CS-1, hCS, PL.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Placental Lactogen Caprine Recombinant, is a single polypeptide chain containing 199 amino acids and an additional Ala at the N-terminus having a molecular mass of 23 kDa.
Placental Lactogen Recombinant Goat is purified by proprietary chromatographic techniques.
Placental Lactogen Recombinant Goat is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Placental Lactogen, also known as chorionic somatomammotropin, is a polypeptide hormone produced by the syncytiotrophoblasts of the placenta. It exhibits both growth hormone and prolactin activities, influencing growth, lactation, and luteal steroid production. In pregnant women, placental lactogen secretion commences shortly after implantation and can reach levels of 1 gram or more per day during late pregnancy. Notably, placental lactogen also acts as an insulin antagonist. It's important to note that Goat Placental Lactogen can activate human and other heterologous GH receptors, but not ruminant GH receptors.
Description
Recombinant Caprine Placental Lactogen is a single polypeptide chain consisting of 199 amino acids, with an additional alanine residue at the N-terminus, resulting in a molecular weight of 23 kDa. The purification process of this recombinant protein involves proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile-filtered.
Formulation
The protein solution, at a concentration of 1 mg/ml in 0.02-0.03% NaHCO3, undergoes a lyophilization process.
Solubility
For reconstitution of the lyophilized Placental Lactogen, sterile 18 MΩ-cm H2O is recommended, with an initial concentration of not less than 100 µg/ml. Further dilutions can be made using other aqueous solutions.
Stability
Lyophilized Recombinant Caprine Placental Lactogen demonstrates stability at room temperature for a period of 3 weeks. However, for extended storage, it is advised to store the lyophilized product in a desiccated environment below -18°C. Upon reconstitution, Placental Lactogen (Goat) should be stored at 4°C for a period of 2-7 days. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. It's crucial to avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 98.0%, as determined by: (a) SEC-HPLC analysis. (b) SDS-PAGE analysis.
Biological Activity
Caprine Placental Lactogen exhibits biological activity, as evidenced by its ability to induce proliferation in Nb2 cells.
Protein Content
Protein content is determined using UV spectroscopy at 280 nm. An absorbance value of 0.85 is used as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated using the PC GENE computer analysis program for protein sequences (IntelliGenetics).
Synonyms
Chorionic Somatomammotropin Hormone 1, CSH1, CS-1, hCS, PL.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Glu-Asn-Tyr.
Product Science Overview
Structure and Composition
Production and Purification
Biological Activity
Placental Lactogen Caprine Recombinant is biologically active and has been shown to induce the proliferation of Nb2 cells, indicating its functional similarity to natural placental lactogen . It is also capable of activating human and other heterologous growth hormone receptors but not ruminant growth hormone receptors .
Stability and Storage
The lyophilized form of Placental Lactogen Caprine Recombinant is stable at room temperature for up to three weeks. However, for long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. To prevent degradation, it is recommended to add a carrier protein such as 0.1% HSA or BSA and avoid freeze-thaw cycles .
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