Placental Lactogen Bovine
Placental Lactogen Bovine Recombinant
Cat. No.
BT29101
Source
Escherichia Coli.
Synonyms
Chorionic Somatomammotropin Hormone 1, CSH1, CSB, CS-1, hCS-B, BPL, BPLP-I.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 99.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Placental Lactogen Bovine Recombinant, is a single polypeptide chain containing 199 amino acids and an additional Ala at the N-terminus having a molecular mass of 23 kDa.
Placental Lactogen Recombinant is purified by proprietary chromatographic techniques.
Placental Lactogen Recombinant is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Placental lactogen, also known as chorionic somatomammotropin, is a polypeptide hormone produced by the syncytiotrophoblasts of the placenta. This hormone exhibits both growth hormone and prolactin activities, influencing growth, lactation, and luteal steroid production. In women, placental lactogen secretion starts shortly after implantation and can reach levels of 1 gram or more per day during late pregnancy. Notably, placental lactogen also acts as an insulin antagonist. Bovine placental lactogen demonstrates the ability to activate human and other heterologous growth hormone receptors, but not ruminant growth hormone receptors.
Description
Recombinant Bovine Placental Lactogen is a single polypeptide chain with a molecular weight of 23 kDa. It consists of 199 amino acids and an additional alanine residue at the N-terminus. The purification process of this recombinant protein involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a 1 mg/ml solution containing 0.02-0.03% sodium bicarbonate.
Solubility
To reconstitute the lyophilized Bovine Placental Lactogen, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Recombinant Bovine Placental Lactogen remains stable at room temperature for up to 3 weeks. However, it is recommended to store the lyophilized product desiccated at a temperature below -18°C. After reconstitution, the Placental Lactogen solution should be stored at 4°C for 2-7 days. For long-term storage, freezing the solution below -18°C is advised. To enhance stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is recommended. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the protein is greater than 99.0%, as determined by Size Exclusion Chromatography-High Performance Liquid Chromatography (SEC-HPLC) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analyses.
Biological Activity
Bovine Placental Lactogen exhibits biological activity, as demonstrated by its ability to induce proliferation in Nb2 cells.
Protein Content
Protein content was determined using UV spectroscopy at a wavelength of 280 nm. An absorbance value of 0.86 was used as the extinction coefficient for a 0.1% (1 mg/ml) solution at pH 8. This extinction coefficient was calculated using the DNAman computer analysis program based on protein sequence analysis.
Synonyms
Chorionic Somatomammotropin Hormone 1, CSH1, CSB, CS-1, hCS-B, BPL, BPLP-I.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Glu-Asp-Tyr-Ala-Pro.
Product Science Overview
Structure and Composition
Biological Activity
This hormone exhibits both growth hormone (GH) and prolactin-like activities. It is known to induce the proliferation of Nb2 cells, which is a measure of its biological activity . Placental lactogen is also an insulin antagonist, which means it can interfere with insulin’s action, potentially affecting glucose metabolism .
Production and Purification
Stability and Storage
Lyophilized Placental Lactogen Bovine Recombinant is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Once reconstituted, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. To prevent degradation, it is advisable to add a carrier protein such as 0.1% HSA or BSA and avoid freeze-thaw cycles .
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