PLA2G5 Human

Secreted Phospholipase A2-V Human Recombinant
Cat. No.
BT22124
Source
Escherichia Coli.
Synonyms
Calcium-dependent phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, PLA2-10, Group V phospholipase A2, GV-PLA2, MGC46205, hVPLA(2), DKFZp686C2294, sPLA2-V, PLA2G5.
Appearance
Lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Secreted Phospholipase A2-V Human Recombinant was produced with N-terminal His-Tag. PLA2G5 His-Tagged Fusion protein is 15.5 kDa containing 118 amino acid residues of the human secreted phospholipase A2-V and 16 additional amino acid residues – His-Tag (underlined).

Product Specs

Introduction
Phospholipase A2 (PLA2) enzymes play a crucial role in hydrolyzing membrane glycerophospholipids, particularly at the sn-2 position. This action releases arachidonic acid (AA), a key precursor for eicosanoids like prostaglandins and leukotrienes, which are involved in inflammation and other biological processes. PLA2 also generates lysophosholipids, another type of lipid mediator. Among the various PLA2 families, the secretory PLA2 (sPLA2) family, comprising 10 identified isozymes, stands out. These low molecular weight enzymes require calcium ions (Ca2+) for their activity and are implicated in diverse biological processes, including the modulation of eicosanoid production, inflammation, and host defense mechanisms. Studies suggest that sPLA2 may contribute to atherosclerosis by hydrolyzing phosphatidylcholine (PC) within lipoproteins in the arterial wall. This process liberates lyso-PC and free fatty acids, potentially promoting the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic lesions. Research in mice has shown a significant influence of sPLA2 expression on HDL particle size and composition. These studies demonstrate that sPLA2 induction is necessary for the reduction of plasma HDL cholesterol levels in response to inflammatory stimuli. Furthermore, instilling bacteria into the bronchi of rats led to surfactant degradation and a change in the ratio of large to small surfactant aggregates, highlighting the role of sPLA2 in this process.
Description
Recombinant Human Secreted Phospholipase A2-V was produced with an N-terminal His-Tag. The PLA2G5 His-Tagged Fusion protein has a molecular weight of 15.5 kDa. It consists of 118 amino acid residues from the human secreted phospholipase A2-V and an additional 16 amino acid residues for the His-Tag (underlined).
Physical Appearance
The product appears as a lyophilized (freeze-dried) powder.
Formulation
The product has been filtered (0.4um) and lyophilized in a solution of 0.5 mg/mL in 0.05M Acetate buffer at pH 4.
Solubility
To prepare a working stock solution of approximately 0.5 mg/mL, add 0.1M Acetate buffer at pH 4 to the lyophilized pellet and allow it to dissolve completely. If you need to adjust the pH to a higher value, we recommend diluting the solution significantly with the appropriate buffer to a concentration of 10 µg/ml. This antigen's solubility is limited at higher concentrations. Please note that this product is not sterile. Before using it in cell culture, filter the product through an appropriate sterile filter.
Stability
For long-term storage, keep the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freezing and thawing cycles. The reconstituted protein can be stored at 4°C for a short period; it remains stable for at least two weeks at this temperature.
Purity
SDS-PAGE analysis indicates a purity greater than 95%.
Synonyms
Calcium-dependent phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, PLA2-10, Group V phospholipase A2, GV-PLA2, MGC46205, hVPLA(2), DKFZp686C2294, sPLA2-V, PLA2G5.
Source
Escherichia Coli.
Amino Acid Sequence

MRGSHHHHHH GMASHMGLLD LKSMIEKVTG KNALTNYGFY GCYCGWGGRG TPKDGTDWCC WAHDHCYGRLEEKGCNIRTQ SYKYRFAWGV VTCEPGPFCH VNLCACDRKL VYCLKRNLRS YNPQYQYFPN ILCS.

Product Science Overview

Introduction

Secreted Phospholipase A2 (sPLA2) enzymes are a group of extracellular enzymes that hydrolyze phospholipids to release free fatty acids and lysophospholipids . Among the various isoforms, Secreted Phospholipase A2-V (sPLA2-V) plays a significant role in various biological processes, including inflammation, metabolism, and cancer .

Structure and Function

sPLA2-V is characterized by its low molecular mass and requirement for calcium ions (Ca2+) for its enzymatic activity . It contains a conserved His-Asp catalytic dyad, which is crucial for its function . The enzyme hydrolyzes the sn-2 position of glycerophospholipids, leading to the production of bioactive lipid mediators such as prostaglandins and leukotrienes .

Biological Roles

sPLA2-V is involved in several physiological and pathological processes:

  • Inflammation: sPLA2-V has been implicated in the inflammatory response by promoting the release of pro-inflammatory lipid mediators .
  • Metabolism: It plays a role in metabolic disorders such as obesity, diabetes, and insulin resistance by modulating lipid metabolism .
  • Cancer: sPLA2-V is involved in cancer-related inflammation and angiogenesis by inducing the release of proangiogenic and antiangiogenic factors .
Research and Applications

Recent studies have highlighted the importance of sPLA2-V in various biological events. For instance, research has shown that sPLA2-V induces the release of vascular endothelial growth factor (VEGF)-A and other angiogenic factors, which are crucial for tumor growth and metastasis . Additionally, sPLA2-V has been studied for its potential therapeutic applications in treating inflammatory and metabolic disorders .

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