PLA2G1B Human
Secreted Phospholipase A2-IB Human Recombinant
Cat. No.
BT21783
Source
Escherichia Coli.
Synonyms
Phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Group IB phospholipase A2, PLA2, PLA2A, PPLA2, sPLA2-IB,MGC119834, MGC119835, PLA2G1B.
Appearance
Lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Secreted Phospholipase A2-IB Human Recombinant is manufactured with N-terminal fusionf HisTag. PLA2G1B His-Tagged Fusion Protein is 16 kDa containing 126 amino acid residues of the human secreted phospholipase A2-IB and 16 additional amino acid residues - HisTag (underlined).
Product Specs
Introduction
Secretory phospholipase A2-IB (sPLA2-IB) belongs to Group IB and plays a role in cell proliferation, migration, hormone release, and eicosanoid production by interacting with its receptor in peripheral tissues. High-affinity binding sites for sPLA2-IB have been identified in the CNS. sPLA2-IB has been shown to cause concentration-dependent neuronal cell death, a process influenced by PGD2 metabolites, particularly Delta12-PGJ2, which may contribute to sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family comprises ten identified isozymes. These are low molecular weight, Ca2+-dependent secretory enzymes involved in various biological processes such as modulating eicosanoid synthesis, inflammation, and host defense.
Description
Recombinant Human Secreted Phospholipase A2-IB is produced with an N-terminal HisTag. The PLA2G1B His-Tagged Fusion Protein has a molecular weight of 16 kDa and consists of 126 amino acids from human secreted phospholipase A2-IB, along with an additional 16 amino acid HisTag sequence (underlined).
Physical Appearance
The product is provided as a lyophilized powder.
Formulation
The product has been sterile filtered (0.4 µm) and lyophilized from a 0.5 mg/ml solution in 0.05 M Acetate buffer at pH 4.
Solubility
To prepare a working stock solution of approximately 0.5 mg/mL, reconstitute the lyophilized powder in 0.1 M Acetate buffer at pH 4. Allow sufficient time for the pellet to dissolve completely. For use at higher pH values, dilute the stock solution to a concentration of 10 µg/ml using the appropriate buffer. The solubility of this antigen is limited at higher concentrations. Please note that this product is not sterile. Filter the product through a sterile filter before using it in cell culture.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to avoid repeated freeze-thaw cycles. The reconstituted protein can be stored at 4°C for a limited time and shows no significant change after two weeks at this temperature.
Purity
The purity of the product is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
Phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Group IB phospholipase A2, PLA2, PLA2A, PPLA2, sPLA2-IB,MGC119834, MGC119835, PLA2G1B.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASHMAVWQ
FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS
YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS.
Product Science Overview
Structure and Mechanism
sPLA2-IB is a low molecular weight, calcium-dependent enzyme characterized by a conserved His-Asp catalytic dyad . The enzyme catalyzes the hydrolysis of phospholipids, leading to the production of bioactive lipid mediators such as arachidonic acid, which are precursors for prostaglandins and leukotrienes . These lipid mediators play crucial roles in inflammatory responses and other cellular processes .
Biological Roles
sPLA2-IB is involved in a variety of biological functions:
- Inflammation: sPLA2-IB has been implicated in the inflammatory response by catalyzing the first step of the arachidonic acid pathway . This pathway leads to the production of several inflammatory and thrombogenic molecules .
- Atherosclerosis: The enzyme contributes to the hydrolysis of low-density lipoprotein (LDL), resulting in the formation of pro-atherogenic LDL, which promotes macrophage foam cell formation .
- Metabolic Regulation: Recent studies have shown that sPLA2-IB plays a role in metabolic disorders such as obesity, hepatic steatosis, diabetes, insulin resistance, and adipose tissue inflammation .
Research and Applications
Research on sPLA2-IB has been facilitated by the use of transgenic and knockout mouse models, which have helped to elucidate the enzyme’s distinct and unique roles in various biological events . Additionally, human recombinant versions of sPLA2-IB are used in research to study its structure, function, and potential therapeutic applications.
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